Bücher veröffentlicht von Springer-Verlag New York Inc.

This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.

This book illustrates experimental and computational methodologies used to achieve cost effective biological processes for the production of fuels and biochemicals through multiple approaches to increasing yield, titers, and productivity in a robust host. The volume includes the most recent and cutting-edge aspects of pathway engineering, flux analysis, and metabolic enzyme engineering. Each chapter highlights the complexity and challenges of the problem as well as the methods used to solve this problem or changes needed in current methods. As a part of the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that gives researchers a much needed boost. Authoritative and practical, Metabolic Pathway Engineering benefits not only scientists working on more fundamental aspects of this endeavor but also those in the biochemical industry working on strain engineering for robust industrial processes.

This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resourcefor both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.

Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophils: Methods and Protocols, chapters retained from previous editions have been have been updated and include many new approaches. In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps. Neutrophil Methods and Protocols: Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.Features¿ Provides neutrophil protocols that are broadly applicable to leukocyte biology¿ Includes protocols for isolation of neutrophils from humans and animal species¿ Presents an interesting and informative overview of the field of neutrophil biology¿ Facilitates a better understanding of the role of neutrophils in host defenseReadershipMolecular and Cell Biologists, Immunologists, Infectious Disease Specialists, Microbiologists

This volume focuses on computational modeling of the ecotoxicity of chemicals and presents applications of quantitative structure¿activity relationship models (QSARs) in the predictive toxicology field in a regulatory context. The extensive book covers a variety of protocols for descriptor computation, data curation, feature selection, learning algorithms, validation of models, applicability domain assessment, confidence estimation for predictions, and much more, as well as case studies and literature reviews on a number of hot topics. Written for the Methods in Pharmacology and Toxicology series, chapters include the kind of practical advice that is essential for researchers everywhere. Authoritative and comprehensive, Ecotoxicological QSARs is an ideal source to update readers in the field with current practices and introduce to them new developments and should therefore be very useful for researchers in academia, industries, and regulatory bodies.

This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ion Mobility-Mass Spectrometry: Methods and Protocols serves as anideal resource for scientists delving into the technique's unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples.

In this soon-to-be gold-standard text, a distinguished panel of experts provides a comprehensive discussion of the various medical, neurological, psychiatric, and psychological factors underlying fatigue and sleepiness in the workplace and in patients. In reviewing current best practices in managing fatigue and sleepiness conditions to improve workplace safety, the book importantly begins with an overview of fatigue's definitions and concepts. It then moves into discussing the historical perspective on sleepiness and fatigue, the definitions and classifications of fatigue, and assessments of fatigue in the laboratory and in clinical settings. The book continues by exploring medical causes of fatigue in detail; how medication and recreational agents may result in fatigue; and the treatment of sleep disorders. Additional topics include best practices in the treatment of narcolepsy and hypersomnia, a review of circadian rhythm and fatigue, the treatment of heart failure and improvement in fatigue, and the management of fatigue in hospitalized patients. A major contribution to the field, this invaluable title will be of significant interest to all clinicians concerned with safeguarding safety in the workplace by accurately diagnosing and managing patients with fatigue and sleepiness problems. Researchers will also find this title of great interest.

The second editon of this popular ultrasound book expands the reader's understanding of the clinical applications of ocular ultrasound through a case study approach. With the addition of high-quality video segments of examination techniques not currently available in any other format, this edition appeals to a broader range of practitioners in the field by presenting the subject starting at the basic level and progressing to the advanced. The book is appealing to practitioners involved in ocular ultrasound, including ophthalmic technicians, ophthalmologists, optometrists, radiologists and emergency room physicians who, on occasion, are involved in the practice of ophthalmic ultrasound.

This contributed volume takes a comprehensive look at factors that impact correctional health care and the related implications for public health and public health policy. It identifies the most compelling health problems behind bars (including communicable and chronic diseases, mental illness, addiction, and suicide), pinpoints systemic barriers to care, and explains how correctional medicine can shift from emergency or crisis care to primary care and prevention. It also discusses the impact of public policy on correctional populations and analyzes the impact on public health as prisoners are released. In this new edition, the multidisciplinary authorship continues to make a timely case for correctional health care that is humane for those incarcerated and beneficial to the communities they re-enter.Keeping in mind that the United States of America leads the world in the percentage of its population that is incarcerated, the book grapples with whether crime in our communities is diminished by incarcerating more and more people and whether health care behind bars could improve the health status of our communities. Special concerns arise when there are prisoners with physical or mental disabilities, who have spent long periods in segregation, and others who are simply growing old.New to the second edition are chapters on correctional nursing, sanitation to prevent intramural transmission, transitions from prisons to communities, the European experience, and root cause analysis for quality improvement, as well as revisions/updates to more than half of the chapters from the first edition that published in 2007.Public Health Behind Bars: From Prisons to Communities, 2nd Edition, should be of immediate interest to correctional health practitioners and correctional administrators. The text also is essential reading for civil rights attorneys, journalists, scholars whose work is at the interface of criminal justice and public health, and students of criminal justice, public health, community health, healthcare administration, health policy, civil rights law, and sociology."In 2007, when Robert Greifinger first compiled a trove of information about the distressing intersection of public health and incarceration, it was, he says, like a textbook for a class that didn't exist. Since then the physical and mental health care crisis in our prisons and jails has aroused a national sense of urgency, and this extensively updated collection of solutions-based essays could not be more timely. It is an invaluable resource for policy-makers, educators, reform activists ¿ and journalists". ¿ Bill Keller, Founding Editor, The Marshall Project, New York, NY, USA

This book brings together a collection of methods for the successful detection, culture and investigation of H. pylori, and its associated diseases. Chapters guide the reader through overviews on H. pylori infection, protocols for analyzing human clinical samples, and protocols for characterizing H. pylori, its functional components and its interactions with host cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Helicobacter pylori: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

Providing a comprehensive presentation of the diagnosis, evaluation and management of malunions, this generously illustrated text details the current principles, surgical techniques and approaches with these challenging clinical situations. Since each malunion can be fairly specific, the treatment regimens provide guidelines to effectively approach such problems. Opening with a brief overview of the principles of malunions, the remainder of the book is divided by anatomical area and provides evidence-based recommendations, case examples, and preferred treatment/algorithms for both the upper and lower extremities, including the clavicle, proximal and distal humerus, hand and wrist, proximal and distal femur, and tibia and ankle, as well as the pelvis and acetabulum. Specialized circumstances are also discussed, including periprosthetics and joint replacement.Although not every single treatment option is described for every single anatomical area and type of injury, Malunions is an excellent resource for orthopedic trauma surgeons, residents and students, not only for managing these common yet complex problems but also in preventing malunions from occurring by avoiding surgical causes and mitigating patient risk factors.

When the first edition of Vascular Reconstructions was published almost twenty years ago, endovascular surgery was still in its infancy. Endovascular approaches became the preferred initial intervention for many vascular pathologies resulting in a major decrease in open vascular surgical procedures. In order to build upon changes in the management of vascular pathology over the past two decades, this second edition of Vascular Reconstructions was conceived. The first part starts with a review of commonly used vascular instruments and an overview of grafts and sutures used in vascular reconstructions. A detailed chapter on vascular anatomy and exposures is provided to serve as a quick reference before starting a vascular procedure. The remaining chapters in the first part of this book review the basic steps usually performed before and after constructing a vascular anastomosis in addition to thrombectomy and endarterectomy. The second part of this book focuses on the various methods used to conduct a vascular reconstruction, which include primary closure, closure with a patch angioplasty, end-to-end, end-to-side, and side- to-side anastomoses. The various possible modifications used are outlined. The third part of this book reviews the various adjunctive methods used when constructing the proximal or distal anastomoses of an infrainguinal bypass. The fourth part of the book reviews the various modifications that are carried out when constructing the proximal and distal anastomoses of an aortic occlusive or aneurysmal pathology. The vascular anastomoses workshop that inspired the conception of this book is included as an appendix. The fifth part focuses on endovascular therapy and imaging.Vascular Reconstructions: Anatomy, Exposures, and Techniques 2nd Edition aims to make the vascular rotation a pleasant experience for surgical residents, the vascular faculty, recent graduates embarking on conducting vascular reconstructionsindependently, and healthcare providers who wish to be familiar with the various steps involved in conducting a vascular procedure.

This volume describes a range of methods used in complement laboratories and how to interpret the data. Chapters detail methods for depletion of IgG and IgM, quantification of complement proteins, C3dg quantification, complement C3 deposition on endothelial cells, anti-C1q auto-antibodies, and methods for assessment of interactions of proteins with heme. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and clearly written, The Complement System: Innovative Diagnostic and Research Protocols aims to ensure successful results in the further study of this vital field.