Bücher von Ayah Rebhi Hilles

Lo scopo di questo studio è confrontare l'effetto anti-fertilità dell'estratto acquoso di semi di fieno greco (FSA) con quello delle pillole contraccettive orali combinate (COCP) in commercio su ratti femmina, determinando le concentrazioni di ormoni riproduttivi nel siero e osservando l'istologia del loro tessuto uterino, determinando lo spessore dell'endometrio uterino, il peso uterino e il tasso di impianto. La somministrazione di 0,05 di COCP ha portato a ridurre la concentrazione sierica di FSH a 71,56 ng/ml, mentre 750 mg/kg di estratto di FSA l'hanno portata a 25,62 ng/ml, il che dimostra che l'estratto di FSA è efficace tre volte di più rispetto ai COCP. Lo spessore dell'endometrio dell'utero nel gruppo trattato con l'estratto di FSA era normale, mentre nei COCP era aumentato. Il peso dell'utero nel gruppo trattato con estratto di FSA era normale, mentre nel gruppo trattato con COCPs era insignificante. I tessuti dell'utero nel gruppo trattato con l'estratto di FSA erano normali, mentre nel gruppo trattato con i COCP si sono verificati cambiamenti istopatologici nei tessuti uterini; la distribuzione delle ghiandole è diminuita, il che è un segno notevole di endometrio atrofico, e sono state riscontrate cellule decidue anomale (cellule simili alle decidue).

Ziel dieser Studie ist es, die fruchtbarkeitsfördernde Wirkung des wässrigen Extrakts aus Bockshornkleesamen (FSA) mit den auf dem Markt befindlichen kombinierten oralen Verhütungspillen (COCPs) bei weiblichen Ratten zu vergleichen, indem die Konzentrationen der Fortpflanzungshormone im Serum bestimmt und die Histologie des Uterusgewebes, die Dicke der Gebärmutterschleimhaut, das Gewicht der Gebärmutter und die Implantationsrate untersucht werden. Die Verabreichung von 0,05 COCPs führte zu einer Verringerung der FSH-Konzentration im Serum auf 71,56 ng/ml, während 750 mg/kg FSA-Extrakt zu einer Verringerung auf 25,62 ng/ml führte, was zeigt, dass der FSA-Extrakt dreimal so wirksam ist wie COCPs. Die Dicke der Gebärmutterschleimhaut war in der mit FSA-Extrakt behandelten Gruppe normal, während sie bei den COCPs zunahm. Das Gewicht der Gebärmutter war in der mit FSA-Extrakt behandelten Gruppe normal, während es in der mit COCPs behandelten Gruppe nur unwesentlich erhöht war. Das Uterusgewebe in der mit FSA-Extrakt behandelten Gruppe war normal, während in der mit COCPs behandelten Gruppe histopathologische Veränderungen im Uterusgewebe auftraten; die Verteilung der Drüsen nahm ab, was ein bemerkenswertes Zeichen für ein atrophisches Endometrium ist, und es gab abnormale deziduale Zellen (dezidualähnliche Zellen).

El objetivo de este estudio es comparar el efecto antifertilidad del extracto acuoso de semillas de fenogreco (FSA) con las píldoras anticonceptivas orales combinadas (PAOC) comercializadas en ratas hembra mediante la determinación de las concentraciones séricas de hormonas reproductivas y la observación de la histología del tejido uterino, determinando el grosor del endometrio uterino, el peso uterino y la tasa de implantación. La administración de 0,05 de COCPs llevó a disminuir la concentración sérica de FSH a 71,56 ng/ml mientras que 750 mg/kg de extracto de FSA llevaron a disminuirla a 25,62 ng/ml lo que demuestra que el extracto de FSA es eficaz tres veces más que los COCPs. El grosor del endometrio del útero en el grupo tratado con extracto de FSA fue normal, mientras que en el tratado con COCP aumentó. El peso del útero en el grupo tratado con extracto de FSA fue normal, mientras que en el grupo tratado con COCPs fue insignificantemente elevado. Los tejidos uterinos del grupo tratado con extracto de FSA eran normales, mientras que en el grupo tratado con COCPs se observaron cambios histopatológicos en los tejidos uterinos; la distribución de las glándulas disminuyó, lo que constituye un signo notable de endometrio atrófico, y se observaron células deciduales anormales (células similares a las deciduales).

Le but de cette étude est de comparer l'effet anti-fertilité de l'extrait aqueux de graines de fenugrec (FSA) avec les pilules contraceptives orales combinées commercialisées (COCP) sur des rats femelles en déterminant les concentrations sériques d'hormones de reproduction et en observant l'histologie de leur tissu utérin, en déterminant l'épaisseur de l'endomètre utérin, le poids de l'utérus et le taux d'implantation. L'administration de 0,05 de COCPs a conduit à une diminution de la concentration sérique de FSH à 71,56 ng/ml alors que 750 mg/kg d'extrait de FSA a conduit à une diminution à 25,62 ng/ml ce qui montre que l'extrait de FSA est trois fois plus efficace que les COCPs. L'épaisseur de l'endomètre de l'utérus dans le groupe traité par l'extrait de FSA était normale alors qu'elle augmentait dans le groupe traité par les COCPs. Le poids de l'utérus dans le groupe traité par l'extrait de FSA était normal alors qu'il était insignifiant dans le groupe traité par les COCPs. Les tissus utérins du groupe traité à l'extrait de FSA étaient normaux alors que dans le groupe traité aux COCPs, il y avait des changements histopathologiques dans les tissus utérins ; la distribution des glandes diminuait, ce qui était un signe remarquable d'endomètre atrophié et il y avait des cellules déciduales anormales (cellules semblables aux cellules déciduales).

O objetivo deste estudo é comparar o efeito anti-fertilidade do extrato aquoso de sementes de feno-grego (FSA) com pílulas contraceptivas orais combinadas comercializadas (COCPs) em ratas fêmeas, determinando as concentrações séricas de hormonas reprodutivas e observando a histologia do tecido uterino, determinando a espessura do endométrio uterino, o peso uterino e a taxa de implantação. A administração de 0,05 de COCPs levou à diminuição da concentração sérica de FSH para 71,56 ng/ml, enquanto 750 mg/kg de extrato de FSA levou à sua diminuição para 25,62 ng/ml, o que mostra que o extrato de FSA é três vezes mais eficaz do que os COCPs. A espessura do endométrio do útero no grupo tratado com extrato de FSA era normal, enquanto que nos COCPs aumentou. O peso uterino no grupo tratado com extrato de FSA era normal, enquanto o grupo tratado com COCPs era insignificantemente elevado. Os tecidos do útero no grupo tratado com extrato de FSA eram normais, ao passo que no grupo tratado com COCPs, havia alterações histopatológicas nos tecidos uterinos; a distribuição das glândulas diminuiu, o que constitui um sinal notável de endométrio atrófico, e havia células deciduais anormais (células semelhantes a deciduais).

The purpose of this study is to compare the anti-fertility effect of fenugreek seeds aqueous (FSA) extract with marketed combined oral contraceptive pills (COCPs) on female rats by determining serum reproductive hormone concentrations and observing their uterus tissue histology, determining the thickness of endometrium uterus, the uterine weight and the implantation rate. Administration of 0.05 of COCPs led to decrease the serum FSH concentration to 71.56 ng/ml while 750 mg/kg FSA extract led to decrease it to 25.62 ng/ml which shows that FSA extract is effective three times more than COCPs. The endometrium thickness of the uterus in the FSA extract treated group was normal while in the COCPs increased. The uterine weight in the FSA extract treated group was normal while the COCPs treated group was insignificantly high. The uterus tissues in the FSA extract treated group was normal while in the COCPs treated group, there were histopathological changes in the uterine tissues; the glands distribution decrease which a remarkable sign of atrophic endometrium and there were abnormal deciduiod cells (decidual like cells).

Asian swamp eel (Monopterus albus) has a unique morphological elongated body which is similar to the snake and covered with a thick layer of the mucus. This study aimed to investigate the anti-oxidative, anti-cancer and antimicrobial activities of eel skin mucus in-vitro. The antioxidant results revealed that methanol extract showed higher activity than aqueous extract with higher phenolic and flavonoid contents as well as higher antioxidant assays which including DPPH radical scavenging and ¿-carotene bleaching. Preliminary cytotoxicity study was demonstrated against human lung carcinoma cell line (A549) using cell viability assay which revealed that methanol extract is more potent than aqueous extract as IC50 values were 621±0.09 µg/mL and 845 ± 0.25 µg/mL respectively. Assessment of antimicrobial activity of the eel mucus extracts against several bacterial and fungal strains was conducted using diffusion method. Methanol extract at 100 µl/well was found to inhibit the growth of Microsporum gypseum and Aspergillus niger. Significantly. Furthermore, the methanol extract at 100 µl/disc significantly inhibited the growth of Staphylococcus aureus and Escherichia coli.

Asian swamp eel is a freshwater eel covered with a thick layer of mucus. The aim of this study is to evaluate the therapeutic activity of eel skin mucus in-vivo. For in-vivo study, a topical gel delivery system used from eel skin mucus formulated gel to apply into the infected rat skins. Sprague Dawley rats were divided into normal, positive control, negative control, and treated groups. The infections were introduced to the rats by intracutaneous injections of pathogenic bacteria and fungi. The development of impetigo, tinea capitis and cutaneous candidiasis in the animal model was confirmed based on the clinical and histological observations. Following that, the infected rats were treated topically with the formulated gel from eel mucus extract. The histological analysis of the skin tissues which treated with the formulated gel was shown a complete recovery in the skin tissues at a similar rate as the control antibiotic groups. In conclusion, the present study revealed that eel skin mucus formulated gel efficient therapeutic candidate in treating skin infections which can be considered as a novel discovery as a natural alternative treatment for certain skin diseases.