Bücher von Feng Zhang

This book constitutes the proceedings of the 24th International Conference on Web Information Systems Engineering, WISE 2023, held in Melbourne, Victoria, Australia, in October 2023.The 33 full and 40 short papers were carefully reviewed and selected from 137 submissions. They were organized in topical sections as follows: text and sentiment analysis; question answering and information retrieval; social media and news analysis; security and privacy; web technologies; graph embeddings and link predictions; predictive analysis and machine learning; recommendation systems; natural language processing (NLP) and databases; data analysis and optimization; anomaly and threat detection; streaming data; miscellaneous; explainability and scalability in AI.

This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.