Bücher der Reihe Colloquium Series on Quantitative Cell Biology

Introduces quantitative microscopy and mathematical modelling approaches that have been used to count the copy number of endocytic proteins, infer their localization with nanometer precision, and infer molecular and physical mechanisms that are involved in the robust formation of endocytic vesicles.

Cells are inherently physical entities that both experience mechanical forces from their external environment and generate their own internal forces to drive cell motion. The aim of this volume is to present the reader with an introduction to the primary tools used to measure these mechanical interactions and the material properties of cells that result from them.

A fundamental component of quantitative cell biology is the ability to count molecules within cells. The numbers of molecules and stoichiometries are the basis for structural models of protein complexes and simulations of biological processes. A variety of methods exist for in vivo quantifications, but the focus of this volume is mainly on fluorescence methods.