Bücher der Reihe Methods in Molecular Biology

This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression.

This volume presents an overview of contemporary quantitative proteomics methods along with instructions on data interpretation, while providing examples on how to implement proteomics into systems biology.

This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques.

This volume includes a series ofprotocols focused on mitotic spindle assembly and function. The methods coveredin this book feature a broad range of techniques from basic microscopy to thestudy of spindle physiologies relevant to cancer. These methods can be appliedto diverse model systems that range from the cell-free Xenopus eggextract system to the moss Physcomitrella patens, in an effort todemonstrate the key contributions made by researchers using multiple modelorganisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to thecross-disciplinary efforts, such as ATP analogue sensitive inhibition ofmitotic kinases. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics,lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips ontroubleshooting and avoidingknown pitfalls.Thorough and informative, TheMitotic Spindle: Methods and Protocols, is a valuable resource forresearchers who are new to mitosis or are already experts in the field.

Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease.

Presentingstate-of-the-art protocols to study gap junctions, this detailed book firstfocuses on the use of methods and tools to investigate the different aspects ofconnexin expression and gap junction regulation.

The NuclearReceptor Superfamily, Second Edition will benefit those starting out in thenuclear receptor research field as well as to more established researchers whowish to apply different methods to a particular receptor or research problem.

Thissecond edition details new emerging areas of zebrafish research focusing on geneticsand genomics, techniques for developing and analyzing zebrafish disease models,and methods for neuroscience. Zebrafish:Methods and Protocols, Second Edition guides readers through methods for mutagenesisand genome editing in zebrafish, applicationsof GFP-expressing transgenic fish, techniques for cancer models, imaging of infectionand host-pathogen interactions, metabolism and transport of lipids, and the structure and function of neural circuitsand their role in generating behavior. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls. Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a usefulcomplement to the first book for new and experienced zebrafish researcheralike.

This volumepresents detailed laboratory protocols regarding the three major routetechnologies luciferases, efficient opticalprobes, and applications to visualizing molecular events in living subjects.

This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins.

"Published In: United States, 07 December 2016"--Distributor's website.

This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution.

In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules.

With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples.

In Clinical Bioinformatics, Second Edition, leading experts in the field provide a series of articles focusing on software applications used to translate information into outcomes of clinical relevance.

Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals.

In Osteoporosis and Osteoarthritis expert investigators detail modern tools to enhance discovery in bone and cartilage biology and promote translational research in osteoporosis and osteoarthritis.

This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system).

In The Low Molecular Weight Proteome: Methods and Protocols expert researchers from the field provide protocols for analysis of low molecular weight proteins and peptides, protocols for such methods applied in clinical research and an up-to-date review of quantitative protein profiling by labeling.

This second edition of Nanobiotechnology Protocols expands upon the previous editions with current, detailed protocols for nanobiotechnology; imaging and detection. It includes new chapters that explore nano-bio constructs and toxicology of nanomaterials.

This book details various aspects of nanotechnology from the oxidative stress point of view, with a focus on synthesis of different antioxidant nanoparticles and antioxidant-loaded nanoparticles, as well as their in vitro/ in vivo mechanisms of action.