Bücher der Reihe Methods in Molecular Biology

Chapters guide the readers through exploiting a number of protein scaffolds including virus nanoparticles, elastin and collagen peptides and proteins, and other protein templates for either building materials or presentation of ligands.

This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that focus on a variety of amyloids and how to study these peptides and proteins. Part Two describes cell culture models and assays, and Part Three explores methods on how to extract amyloid from tissue, its detection, and its characterization in vitro or in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Amyloid Proteins: Methods and Protocols, Third Edition is a valuable resource for both students and scientists who are new to the field, as well as experienced researchers who would like to progress their research with the latest available techniques.

This volume expands on the previous edition with a more extensive look at molecular motors and their roles in muscle contractions, vesicle transport, flagellar beating, chromosome segregation, and DNA replication and repair. The chapters in this book are divided into three parts: Part One looks at membrane motors, such as the bacterial flagellar rotary motor; Part Two discusses cytoskeletal motors, such as kinesin and myosin; and Part Three talks about nucleic acid motors, such as DNA polymerases, helicases, and nucleosome remodelers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Molecular Motors: Methods and Protocols, Second Edition is a valuable resource for (bio)physicists and molecular/cellular biologists whose research delves into the mechanisms at work in cells and the motors which power them.

This volume features a comprehensive set of protocols featuring a range of both old and new technologies that can be used to analyze drugs of abuse, including prescription drugs, new psychoactive substances and psychoactive plants.

This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations.

This volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study.

Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication.

In Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study mouse embryology.

PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one's own tests or introducing tests developed by other workers.

This book presents a compendium of advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and vascular biology research. It details classical methods as well as new protocols and strategies.

A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases.

Computer-Aided Tissue Engineering highlights the interdisciplinary nature of this topic and reviews the current state of computer-aided three-dimensional tissue modeling, tissue classification, and tissue fabrication and implantation.

Over the last two decades advances in genotyping technology, and the development of quantitative genetic analytical techniques have made it possible to dissect complex traits and link quantitative variation in traits to allelic variation on chromosomes or quantitative trait loci (QTLs).

This Methods in Molecular Biology(TM) volume covers experimental and theoretical methodology of fluorescence properties, application of fluorescence spectroscopy, single molecule approaches, and electro-optical analysis of macromolecular structure and dynamics.

This Methods in Molecular Biology(t) volume offers selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Includes materials and reagent lists, step-by-step protocols, troubleshooting advice and more.

Now in its Third Edition, this Methods in Molecular Biology(TM) book offers a thorough guide to reproducible plant cell culture protocols, including lists of materials and reagents, tips on troubleshooting and avoiding known pitfalls, and much more.

Systems biology is the study of interactions between assorted components of biological systems with the aim of acquiring new insights into how organisms function and respond to different stimuli.

Focusing on Systems of ODEs, parameterization of signaling models, signaling pathways, mass-action kinetics and ODEs and how to use modeling to plan experiments, this Methods in Molecular Biology(TM) book details steps for getting optimal laboratory results.

This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets.

This book focuses on methods for human clinical practice. It examines assays that are of established or potential clinical utility and are likely to be included in the repertoire of tests provided by a routine diagnostic and service laboratory.

This book details the protocols used for a wide range of experiments in retinal research, including manipulating gene expression in vivo, retinal regeneration and stem cell-based replacement, and non-invasive imaging techniques such as electroretinography.

To aid the process of investigation, the Methods Molecular Biology series has brought together many protocols and has highlighted the useful variations and the pitfalls of the different methods. This information, though usually available in the literature, is often widely dispersed and frequently occurs in older volumes of journals;

Over the last decade the development of new molecular biology tools, advanced microscopy, live imaging and systems biology approaches have revolutionized our conception of how embryonic development proceeds.

Now in a second, revised edition, this practical companion to the methodologies of researching cancer growth includes topical introductions and stepwise, reproducible protocols for laboratory processes, as well as invaluable troubleshooting tips.

A volume in Springer's respected series outlining practical methodologies in a range of disciplines, this book includes stepwise, lab-ready protocols, expert tips and troubleshooting advice, providing the latest commentary on this promising medical technology.