Bücher der Reihe Methods in Molecular Biology

The discipline of developmental toxicology is an integration of concepts, models, and methodologies based heavily on the superimposition of toxicology principles upon the science of developmental biology.

Population genomics is a recently emerged discipline, which aims at understanding how evolutionary processes influence genetic variation across genomes.

The emerging field of prenatal gene therapy is founded on scientific and technical advances in fetal medicine, molecular biology and gene therapy.

Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.S. military and relatively large numbers of U.S. civilians.

While protein identification is an almost routine requirement nowadays, reliable techniques for quantifying unmodified proteins (including those that escape detection under standard conditions, such as protein isoforms and membrane proteins) is not routine.

A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function.

Emphasizing the newest developments in the field, this volume presents detailed methods with added emphasis on therapeutic protein discovery. It features key tips and valuable implementation advice to ensure successful results.

Over the past decade, there has been an explosive development of research of intrinsically disordered proteins (IDPs), which are also known as unfolded proteins.

The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments.

This Methods in Molecular Biology(TM) volume covers LC-MS screening for drugs of abuse, as well as targeted measurement of analytes including abused drugs, toxic compounds, and therapeutic agents. Includes step-by-step protocols, tips and troubleshooting notes.

This Methods in Molecular Biology(TM) book surveys the full range of molecular testing for STI, covering DNA extraction methods, sequence analysis, DNA detection and more. Offers materials lists, step-by-step reproducible protocols, tips and troubeshooting notes.

Stem cell mobilization represents a transient increase in the levels of circulating stem and progenitor cells.

These include methods and techniques to identify regulatory RNAs, characterizing the function and expression of regulatory RNAs in bacterial cells, RNA structure prediction, and interactions between regulatory RNAs and proteins.

This Methods in Molecular Biology(TM) book covers all stages of nanoparticle manufacturing, modification, analysis and application. Includes materials lists, step-by-step, readily reproducible laboratory protocols, troubleshooting tips and avoiding pitfalls.

Biomass conversion research is a combination of basic science, applied science, and engineering testing and analysis. In Biomass Conversion: Methods and Protocols, expert researchers in the field detail methods which are now commonly used to study biomass conversion.

The liver governs many critical life functions and has several distinct cell types. This guide to characterizing liver proteome at every level, from global to subcellular and post-transitional, features laboratory-ready protocols and invaluable expert advice.

The second edition of Bioinformatics and Drug Discovery has been completely updated to include topics that range from new technologies in target identification, genomic analysis, cheminformatics, protein analysis, and network or pathway analysis.

The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications;

In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture.

Recent medical advances have opened up exciting new possibilities for antibodies as a key drug modality in diseases including cancer and neurodegenerative disorders. This updated second edition has detailed expert guidance on the latest methods and protocols.

These include methods and techniques of the manipulation of physical forces that shape progenitor cell behavior, studying progenitor cells in vivo, using non-mammalian and mammalian model systems, and investigating human progenitor cells, including their isolation, characterization and application in cell-based therapies.

Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants.

This Methods in Molecular Biology(TM) book is written on the premise that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. Offers materials lists, protocols and troubleshooting tips.

This Methods in Molecular Biology(TM) book addresses special considerations when applying toxicity studies to nanomaterials and details new methods for the study of nanotoxicity. Includes materials lists, step-by-step lab protocols, troubleshooting tips and more.

This Methods in Molecular Biology(TM) book details rational drug design, including virtual screening of chemical hits, lead discovery by high throughput screening, combinatorial and fragment based lead generation, peptide based drug discovery and animal models.

Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers.

This Methods in Molecular Biology(TM) book details approaches to the structure, dynamics and interactions of membrane proteins, including measurement procedures and computational methods for structure prediction and insight on the dynamics of membrane receptors.

This Methods in Molecular Biology(TM) book expands on the thorough coverage of Malaria its previous edition, offering new chapters on parasite culture methods, genome manipulation and more, and offers materials lists, protocols and troubleshooting tips.

This Methods in Molecular Biology(TM) book is an in-depth manual of Capillary Electrophoresis applications in important areas of clinical science, including clinical chemistry, hematology, disease-associated biomarker discovery, immunology and genetic analysis.

This Methods in Molecular Biology(TM) book offers techniques for examining fundamental properties of SSBs and for exploiting the biochemical functions of SSBs as in vitro and in vivo reagents. Includes materials lists, protocols, troubleshooting tips and more.