Bücher der Reihe Methods in Molecular Biology

This Methods in Molecular Biology(TM) book details protocols used in the study of spermatogenesis, including basic and advanced techniques used in clinical andrology laboratories. Includes materials lists, step-by-step protocols and troubleshooting tips.

The final section is a compilation of the key elements and main approaches used in pharmacokinetic and pharmacodynamic modeling, including the modeling of absorption, compartment and non-compartmental modeling, physiologically based pharmacokinetic modeling, interspecies extrapolation, and population effects.

This resource features essential background, context, examples, useful tips, and an overview of current developments in the field. It includes cutting-edge methods and protocols as well as implementation advice from the experts.

This welcome addition to Springer's Methods in Molecular Biology series focuses on the range of methods used to study protein supersecondary structures, and includes authoritative troubleshooting advice alongside its reproducible laboratory protocols.

Detailing the latest protocols for researching animal models of diabetes, especially resistant groups of rodents such as the NOD mouse, and including expert advice on implementation, this is a valuable new volume in the Methods in Molecular Biology series.

As with its stable mates in the Methods in Molecular Biology series, this new book offers reproducible laboratory protocols and expert tips in the field of developing integrative and individualized therapeutics, with applications from drugs to behaviors.

Fully updated and easy to use, this book helps readers explore diverse ways to understand the mechanisms in which miRNAs facilitate the molecular aspects of not only biomedical research but also a wide range of other research fields.

This book explores new protocols and features revised and improved methodologies, making it a valuable reference for all those contemplating a move into the study of intracellular Ca2+. It features lab-ready procedures and includes key implementation advice.

Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply.

Over the past decade, there has been an explosive development of research of intrinsically disordered proteins (IDPs), which are also known as unfolded proteins.

Although many of the new techniques in molecular biology are relatively easy to master, it is often difficult for a researcher to obtain all the relevant information nec- sary for setting up and successfully applying a new te- nique.

Antibacterial Peptide Protocols, will attest, my enthusi asm for the field of antibacterial peptides is based on a conviction (and I am unashamed to say, prejudice) that these substances are in essence antibiotics produced by the host that then participate in host defense against infectious agents.

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells.

For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world.

Neurotransmitter Methods is intended as a bench-side companion for researchers who seek to identify, locaHze, or measure neurotransmitters and/or to identify sites of neurotransmitter action.

Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels.

More specifically, sequencing projects routinely deposit their data in p- licly available databases including GenBank, at the National Center of Biotechnology (NCBI) in the United States; Currently, several browsers offer facile access to numerous genomic DNA sequences for gene mapping and data retrieval.

Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

This book presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function.

This book is dedicated to the characterization of peptides and their applications for the study of biochemical systems. Part II, Application, presents a variety of specific applications for synthetic peptides. This book is an indispensable aid in the pursuit of new directions in peptide research.

Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions.

Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental description, including anecdotal pointers and notes.

This volume provides a collection of robust protocols for molecular biologists studying comparative genomics. Given the tremendous increase in available biosequence data over the past ten years, this volume is timely, comprehensive, and novel. The volume is intended for molecular biologists, biochemists and geneticists.

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively.

This volume both engages the reader and provides a sound foundation for the use of immunoinformatics techniques in immunology and vaccinology. The book contains chapters written by leaders in the field and provides a firm background for anyone working in immunoinformatics in one easy-to-use, insightful volume.

This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders.

Coverage includes applications in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray platforms in clinical practice, and screening of cDNA libraries on glass slide microarrays.

Covering the latest developments in clinical omics, this volume details the algorithms currently used in publicly available software tools. It looks at statistics, algorithms, automated data retrieval, and experimental consideration in the various omics areas.

If one could understand more about the cells that remain after classical chemotherapy or the cells that can remake the tumor among the heterogeneous population, this information would have a huge impact on our treatment of cancer.

In the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche area of study to a major, essential scientific technique.