Bücher der Reihe Methods in Molecular Biology

It has become clear that tumors result from excessive cell proliferation and a corresponding reduction in cell death caused by the successive accumulation of mutations in key regulatory target genes over time.

Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal the unique structural features and biochemical functions of this protein. p53 Protocols comprises eighteen chapters for the study of the diverse properties of p53 and related proteins.

This fully updated and expanded edition details the transgenic techniques currently used to modify the genome. It explores classic procedures as well as cutting-edge practices involving microinjection, site-specific recombination systems, and cryopreservation.

Published In: United States, 23 January 2017"--Distributor's website

This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants.

High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries.--

This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks.

This book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques.

What has emerged over the years is that most chemokine receptors bind several distinct ligands, and indeed the majority of chemokines are able to bind to multiple chemokine receptors, explaining to some extent the apparent disparity in the numbers of chemokines and rec- tors.

This second edition details the newest and most innovative techniques in this rapidly expanding field. It provides methods and assays that can be adapted and used in most specialties of biomedical and biotechnological fields.

Following the completion of the mouse and human genome sequences, a major challengeisthefunctionalcharacterizationofeverymammaliangeneandthedeciph- ing of their molecular interaction network.

The development of the Western blot has revolutionized biomedical research and medical diagnostics. This book offers numerous protein transfer and detection techniques based on the Western blot and includes step-by-step methods and tips on troubleshooting.

Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology.

Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules.

In Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols, experts in the field highlight the main principles and methodologies currently utilized in the study of molecular interactions between compounds, either natural or synthetic, and complementary biological targets, within the scope of drug discovery.

Chemogenomics aims toward the systematic identification of small molecules that interact with the products of the genome and modulate their biological function.

Despite its inherent controversy, the exploration of the human embryo can unlock many answers to deep biological questions. In this book, internationally recognized researchers contribute detailed methods to analyze aspects of the embryogenesis process.

This volume presents the techniques to investigate the critical steps involved in the apoptotic process. Coverage includes detection of apoptosis, modifications of apoptotic proteins during apoptosis, and analysis of apoptosis in different organs.

In Antibody Phage Display expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display.

In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically. In this book, experts provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish.

Made possible by the advent of modern methodology, and ideal for both experienced and novice scientists, this volume provides an up-to-date collection of approaches that can be used to investigate how the mechanism of mitosis operates at the molecular level.

The development and rapid implementation of molecular genotyping methods have re- lutionized the possibility for differentiation and classification of microorganisms at the subspecies level.

This is a fundamental yet comprehensive guidebook for researchers in the field of cancer gene profiling, as well as for those individuals in the area of biomedical/translational research. It examines the world of cancer gene profiling in light of new advances.

In Protein Networks and Pathway Analysis, expert practitioners present a compilation of methods of functional data analysis, often referred to as "systems biology," and its applications in drug discovery, medicine and basic disease research.

In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics.

In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics.

Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise.

Whereas getting exact data about living systems and the sophistication of experimental procedures have primarily absorbed the minds of researchers previously, the weight increasingly shifts to the problem of interpreting accumulated data in terms of biological function and bio- lecular mechanisms.

After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i.

In this book, leading experts provide robust and well-established protocols to elucidate the multiplicity of tools that have been developed to study protein sorting, membrane targeting, transmembrane crossing, and secretion across multiple membranes.