Bücher der Reihe Methods in Molecular Biology

Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body's natural ability to fght disease, heal and recover, or fail and succumb to various maladies.

Due to their rare combination of high chemical stability, exceptional optical and electrical properties, high surface-to-volume ratio, and high aspect ratio, carbon nanotubes (CNTs) have made an enormous impact on materials science, molecular biology, biomedicine, and bioanalytical chemistry.

In this book, leading investigators present a broad, up-to-date collection of current research and experimental methods for the isolation, characterization, production, and optimization of AMPs. The book covers a rapidly expanding field of research.

Early detection of cancer at the cellular level is critical. This book provides the most recent, cutting-edge methods designed to achieve the vital goals of nanotechnology-mediated early cancer detection and less toxic therapies for this devastating disease.

The kingdom Fungi constitutes an independent group equal in rank to that of plants and animals. Fungi are highly amenable to molecular work, and a few fungal species serve as model systems to study basic processes with results that are applicable to many organisms, including humans.

In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning.

The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function.

Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body's own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome.

Photodynamic Therapy

In this timely and authoritative book, career-long experts explore a full range of methods for bioenergy. The volume aims to provide direction and assistance to the growing cadre of researchers endeavoring to develop new sources of bioenergy.

Since the discovery that protein kinase C (PKC) transduces the ab- dance of signals that result in phospholipid hydrolysis, this enzyme has been at the forefront of research in signal transduction. The book ends with a presentation of genetic approaches to study PKC (Part X) and a discussion of approaches used to study PKC in disease (Part XI).

The field of cell cycle regulation is based on the observation that the life cycle of a cell progresses through several distinct phases, G1, M, S, and G2, occurring in a well-defined temporal order.

During the last few years, new discoveries in the RNA field have opened up a wealth of opportunities to specifically target mRNA for functional analysis and target validation. Contained in this volume of Methods in Molecular Biology are methods useful for the design and application of ribozymes, DNAzymes, and small interfering RNAs (siRNAs). In addition, a number of illustrations aiming to facilitate the und- standing and application of each method is included. Like any new field, ribozymes and siRNA research is rapidly evolving, and so too are the methods used to study, select, express, and control their structure and in vivo biological activities. Ribozymes and siRNA Protocols, Second Edition focuses on the latest technical advances in interfering with gene expression. It contains a short int- duction followed by general and specific protocols for using hammerhead ribozymes and derivates, DNAzymes, hairpin ribozymes, group I intron ribozymes, RNase P ribozymes, and siRNAs. Also in this volume are general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy protocols using ribozymes.

This new edition offers updates and important new techniques for illuminating the processes underlying research on metastasis. Presents techniques applicable to living cells and tissues, to cell behavior in vitro, to animal models and mathematical constructs.

Chronic pain is a complex phenomenon. In this book, experts present thorough coverage of molecular analgesia research methods. There are extensive chapters on emerging receptor classes as targets for analgesic drugs and innovative analgesic strategies.

Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism.

In the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche area of study to a major, essential scientific technique.

The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing "no new neuron" doctrine and opened the door to the potential for cell replacement therapy.

In Tiling Arrays: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study tiling microarrays in genomic discovery .

In Peptide Modifications to Increase Metabolic Stability and Activity, expert researchers in the field provide summarized methods for preparation, purification of modified peptides, and assessment of their biochemical activities.

Rev. ed. of: Adenovirus methods and protocols / edited by William S.M. Wold, Ann E. Tollefson. 2nd ed. c2007.

Yet another contribution to the highly successful Methods in Molecular Biology series, this guide to the techniques of bioluminescent imaging has stepwise protocols on cutting-edge methods widely used in research and medicine to track biological processes.

Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.

This volume examines methods for the production and purification of helicases from different species. It also offers detailed studies of helicase activities, including NTP binding and hydrolysis, and translocation along nucleic acid substrates.

Providing crucial information for the expanding ornamental plant industry, leading researchers in the field compile comprehensive and step-wise protocols for rapid plant multiplication and in vitro storage of major commercially viable ornamental plants.

In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components.

Epidermal Cells: Methods and Protocols, Second Edition expands upon the popular first edition by bringing together a panel of experienced basic and clinical researchers to describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells.

This second volume of Advanced Protocols in Oxidative Stress focuses on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data.

This updated book presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. It aims to become a bench-side handbook for both beginners and experts.

When first conceived, not only was the aim of Protocols for Oligo nucleotides and Analogs to provide wide coverage of the ohgonuc- otide chemistry field for readers who are well versed within the field, but also to give investigators just entering into the field a new perspective.