Bücher der Reihe Methods in Molecular Biology

Microchip methods of analysis are now an essential tool for biomedical researchers. Here, the authors focus on DNA microarrays, protein microarrays, and labs-on-chips, and support the introduction of new diagnostic methods based on microtechnologies.

During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis.

Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists.

In Rat Genomics: Methods and Protocols, world-wide experts provide both practical information for researchers involved in genomic research in the rat along with a more contextual discussion about the usefulness of the rat in physiological or translational research in different organs and systems.

With its versatility, ease of use and similarity to the human genome, the mouse has become a top model for medical study. This book provides background of mice as models and details a collection of techniques for a wide variety of specific disease topics.

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix 1 Multidrug Resistance in Cancer . Baguley 2 Multidrug Resistance in Oncology and Beyond: From Imaging of Drug Efflux Pumps to Cellular Drug Targets .

Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists.

In Membrane Transporters in Drug Discovery and Development: Methods and Protocols, expert researchers provide practical methodologies of the ongoing research on membrane transporters, considering applications of transporter technologies in drug discovery and development.

This book is intended for scientifc researchers, clinical laboratorians, clinical and translational scientists, and others interested in proteomics and biomarker discovery.

Plant Secondary Metabolism Engineering

In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues.

This authoritative book emphasizes the molecular effects of protein misfolding at a cellular level, a vital subject. It aims to pinpoint possible manipulations and treatment strategies that can counteract, modify, or delay the consequences of misfolding.

This cutting-edge text will be a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and exciting areas of research such as regenerative medicine and cancer.

Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships. Expert researchers combine the tools of the molecular biologist with those of the microscopist.

Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics.

This Methods in Molecular Biology(TM) volume describes in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. Includes troubleshooting tips and more.

With the recent completion of the sequencing of the human genome, it is widely anticipated that the number of potential new protein drugs and targets will escalate at an even greater rate than that observed in recent years.

A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo.

Here, leading researchers describe the latest techniques for investigating the mechanisms underlying transcription factor nuclear-cytoplasmic trafficking, as well as the functional impact of post-translational modifications on transcription factor function.

In Systems Biology in Drug Discovery and Development: Methods and Protocols, leading experts provide a practical, state-of-the-art, and holistic view of the translation of systems biology into better drug discovery and personalized medical practice.

Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries.

The past decade has seen an extraordinary growth in research interest in neurotrophic factors, and the study of the neurotrophin family has led this activity. Neurotrophin Protocols has brought together many leaders in the neurotrophin field who detail their special expertise in a wide variety of techniques.

Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.

The first few chapters discuss methods for stable delivery of soluble Hh proteins in cell cultures that can be adapted for in vivo studies, and mathematical models for studying Hh proteins gradient formation in tissues.

In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike.

coli vectors and strains for production of pure, functional protein, and the use of E. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.

This book exemplifies recent advances in transcriptional regulation with a set of key cutting-edge protocols from around the world. It explores topics ranging from transcription factors to pre-initiation complex (PIC) assembly and RNA synthesis and regulation.

The purpose of Calpain Methods and Protocols is quite straightf- ward: it is to present the actual experimental methods used in many different laboratories for the study of calpain.

The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line .