Bücher der Reihe Methods in Molecular Biology

In this book, experts from some of the most active laboratories present detailed methods, guidance, and perspectives. It includes coverage of zinc finger nucleases, one of the most exciting tools for gene therapy and genome engineering in the past decade.

Over the past two decades, spectacular advances have been made in our understanding of the molecular genetics of cancer, leading to the pursuit of identifying genes that, when mutated, result in an increased susceptibility to the disease.

This book highlights recent advances in the identification, characterization, and generation of regulatory cells not only of the T cell lineage but also of other origins, as well as the role of several suppressive molecules in immunoregulation.

Chemical library technologies have brought about dramatic changes in the drug discovery process, and, though still evolving, they have become an integral part of ongoing drug discovery research.

Opening with components of the neurovascular unit and the blood-cerebrospinal, blood-retinal, and blood-nerve barriers, this book reviews imaging techniques, molecular study methods, and techniques for delivering therapeutic agents across the BBB.

Exploring the activation and function of components of the MAPK signaling cascades, this volume presents fully updated techniques to study MAPK. Chapters include lists of the necessary materials, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

What has emerged over the years is that most chemokine receptors bind several distinct ligands, and indeed the majority of chemokines are able to bind to multiple chemokine receptors, explaining to some extent the apparent disparity in the numbers of chemokines and rec- tors.

With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis.

In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting;

Thus, a proper understanding of the inflammatory basis may provide clues to new therap- tic targets not only in classical inflammatory diseases, but atherosclerosis, cancer, and ischemic heart disease as well.

Genetic recombination, in the broadest sense, can be defined as any process in which DNA sequences interact and undergo a transfer of information, producing new "recombinant" sequences that contain information from each of the original molecules.

A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease.

The individual chapters now include comp- hensive detailed methods, studies in genetically tractable systems, fluorescence microscopy in live single cells, ex vivo analysis of primary cells from tra- genic mice, as well as genomic and proteomic approaches to the analysis of transmembrane signaling events.

The field of toxicogenomics has been growing quickly. This informative and cutting-edge book collects reviews, opinion pieces and case studies from this dynamic field with a focus on its application to pharmaceuticals.

This volume includes chapters describing, in-depth, the individual steps in the Structural Genomics pipeline, as well as less detailed overviews of individual Structural Genomics initiatives.

Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.

Written in the popular Methods in Molecular Biology(TM) series format, this book presents a collection of techniques aimed at bridging the translational gap in muscle gene therapy between the prevalent rodent models and vitally important larger animal models.

Brings the forefront methods of protein separation and purification directly to your laboratory benchtop. This book treats not only analytical separations, but also the small- and large-scale preparative techniques that are applicable throughout biological and biomedical research. It is suitable for those who are doing protein chromatography.

The field of eukaryotic DNA repair is enjoying a period of remarkable growth and discovery, fueled by technological advances in molecular bi- ogy, protein biochemistry, and genetics.

This revised edition provides up-to-date protocols developed in the HSC field. The book provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations, and offers a variety of transplantation approaches to measure HSC function in vivo.

coli vectors and strains for production of pure, functional protein, and the use of E. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.

Presents detailed outlines of embryonic systems models along with cellular, genetic, and molecular mechanism. This title provides a comprehensive collection of protocols on plan embryo development.

In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components.

MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects.

This book explores imaginative and ambitious multidisciplinary techniques to remove pollutants from a variety of environments. It addresses broader issues surrounding bioremediation and includes detailed protocols of various effective techniques.

Structured in three major sections, this detailed volume covers basic bioinformatics frameworks relating to comparative proteomics, bioinformatics databases and tools for proteomics data analysis, and integrated bioinformatics systems and approaches for studying comparative proteomics in the systems biology context.