Bücher der Reihe Methods in Molecular Biology

Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software.

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration.

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents.

The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer.

MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs.

Neuroinformatics presents cutting-edge techniques for the synergistic study of neuroinformatics. The book facilitates the efforts of discovering neuroscience through the sharing of data and the use of computational models. It contains detailed explanations, advantages, and disadvantages of traditional and non-invasive imaging methods.

For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In this essential book, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs.

As membrane trafficking research has expanded over the past thirty years, a remarkable convergence of information has been gained by using genetic approaches in yeast cells with biochemical approaches in mammalian cells.

Macromolecular drugs could become new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in a volume which gives a wide-ranging overview of contemporary methods used in the field.

This volume on metabonomics provides detailed information on the procedures involved in nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GS-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS).

Proteases contribute decisively to cancer development and promotion. In this volume, expert researchers collate a wide range of current, complimentary techniques developed for the detection and analysis of proteases and their activities in cancer biology.

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents.

The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer.

Microchip methods of analysis are now an essential tool for biomedical researchers. Here, the authors focus on DNA microarrays, protein microarrays, and labs-on-chips, and support the introduction of new diagnostic methods based on microtechnologies.

In this book, expert researchers deliver a systematic guide to techniques addressing various aspects of experimental cancer biology. The book selectively focuses on inflammation-mediated tumorigenesis, while promoting improvisations on a per-case basis.

These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity.

This book presents methods and protocols for both fixed and live cells from everything from bacteria to mammalian models, offering detailed techniques from experts in the field. The text includes vital notes on troubleshooting and avoiding known pitfalls.

Presenting an area of research that intersects with and integrates diverse disciplines, including molecular biology, applied informatics, and statistics, among others, Bioinformatics for Omics Data: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study.

This comprehensive survey of the field sees expert researchers provide concise descriptions of the most up-to-date methodologies used to fabricate microarrays for the analysis of proteins or responses to proteins that help inform our study of human disease.

Part of the respected Methods in Molecular Biology(TM) series, this practical volume features brief introductions to the key topics, lists the necessary materials and reagents, and includes reproducible laboratory protocols as well as key troubleshooting tips.

This book assembles experts from many fields to provide a practical overview of biomedical nanotechnology, from the conception of novel materials in the lab to their application in the clinic. Includes case studies, regulatory and patent information and more.

Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols presents an area of research that intersects with and integrates diverse disciplines. The book's contributions provide practical guidelines to this complex study.

Positron Emission Tomography has revolutionized oncological imaging, and this survey of the field, part of the respected Methods in Molecular Biology(TM) series, seeks to improve the key channels of communication between the imager and the referring specialist.

The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years.

Blood science has become a cornerstone of multiple disciplines. This book, contributed to by leading experts in the field, provides a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics.

Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants.

Yeast Genetic Networks presents an analysis of small-scale gene networks in yeast. The text discusses different methods to analyze gene expression quantitatively, and describes a collection of mathematical and computational tools.

Due to their novel concepts and extraordinary high-throughput sequencing capacity, the "next generation sequencing" methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities.

Atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Covering topics like molecule imaging, nanoscale surface analysis and cellular imaging, this volume features the most up-to-date techniques currently in use.