Bücher der Reihe Methods in Molecular Biology

This Methods in Molecular Biology(TM) volume offers detailed protocols, with theoretical background, and step-by-step instructions on implementing synchronization, as well as current techniques for the enhanced study of regulatory mechanisms in cell cycle events.

This book provides an overview of single-molecule approaches applied to biomolecules, including a solid introduction to the most widely used single-molecule techniques. It features readily reproducible laboratory protocols and notes on troubleshooting.

In Protein Microarrays: Methods and Protocols, expert researchers in the field present an up-to-date collection of robust strategies in the field of protein microarrays and summarize recent advantages in the field of printing technologies, the development of suitable surface materials, as well as detection and quantification technologies.

In this book, experts present a broad range of available and developing techniques for the analysis of gene expression at the DNA, RNA, and protein levels. A collection of clearly described and illustrated chapters includes tips and implementation advice.

This timely and authoritative book aims to provide a comprehensive toolkit to study gene regulatory networks from the point of data generation to processing, visualization, and modeling. The book is intended for novices and experienced researchers alike.

The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form.

This book presents a readily reproducible collection of established and emerging techniques for neuropeptide research. Coverage includes immunocytochemical localization, biochemical characterization, development of genetic probes and functional analysis.

Focusing on the two broad areas of epigenetics, DNA methylation and chromatin modifications, this book highlights select techniques that have been mainstays in the field. It also covers methods especially relevant to extant discoveries in epigenetics.

This volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research.

Despite considerable variability within the scientific community, allosteric regulation can best be defined functionally as how a macromolecule binds one ligand differently when a second ligand is or is not pre-bound to the macromolecule, which constitutes a vital aspect of protein structure/function.

In Kinase Inhibitors: Methods and Protocols, experts in kinase biology, drug discovery, and clinical research present a series of exemplary methods that can be used to address the many challenges facing scientists in the discovery and development of kinase inhibitors both for research and clinical use.

Our understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for research in skeletal muscle biology.

Research into this potentially deadly agent of meningitis and septicaemia is a matter of urgency, and this volume details new methods and technologies that can be deployed to further our understanding of, and ability to tackle, neisseria meningitides.

Avian Influenza Virus, Second Edition aims to provide the essential methods used in working with animal influenza viruses, and to compile more advanced information that will guide the user in designing influenza studies.

The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings.

Cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation. Here, international experts provide up-to-date techniques covering the many key areas necessary for successful transplantation.

Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease.

With the cloning of the green fluorescent protein (GFP) from Aequorea victoria in 1992, another valuable tool was added to the arsenal. In Green Fluorescent Proteins: Applications and Protocols examples of how GFP can be utilized in a variety of fields are presented.

This book presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function.

Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics.

Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species.

Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.).

Understanding the humble seed is a vital goal in molecular biology. This volume, which includes key notes and implementation advice from leading researchers, covers all the latest methods and protocols, including some that have led to major advances.

Androgen Action provides a tool box to study various phases of androgen action, from its entry to the cell, to the phenotypic response that the cell mounts. The text includes up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists.

This volume in the popular Methods in Molecular Biology(TM) series offers an up-to-date collection of methods and approaches for investigating microtubule dynamics in vitro and in cells. Includes material lists, step-by-step protocols and troubleshooting tips.

Cell cycle checkpoints control the fidelity and orderly progression of eukaryotic cell division. This title provides detailed descriptions of methodologies employed by researchers in the field, including those commonly used in the mammalian, yeast, C elegans, Drosophila, and Xenopus model systems.

International biobank collaborations allow for studies with large number of subjects where generalizability of findings across populations can be investigated, which means establishing quality criteria concerning the nature of the sample, conditions of sample storage, and the adequacy of available information is of vital importance.

This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology. In the past 20 years, this field has witnessed a remarkable convergence and synergism from information gained using genetic approaches in yeast cells, and biochemical and molecular approaches in mammalian cells.

This volume explores the considerable efforts that have been directed towards the development of G Protein-Coupled Receptors (GPCR) screening assays in order to disclose GPCR acting compounds, elucidate signaling mechanisms or evaluate compound's efficacy.