Bücher der Reihe Methods in Molecular Biology

This book provides current glycoinformatics methods and protocols used to support the determination of carbohydrate structures in biological samples as well as carbohydrate structure databases, the interaction of carbohydrates with proteins, and theoretical and experimental methods to study their three-dimensional structure and dynamics.

Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists.

Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications.

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E.

Opening with a review of animal research in psychiatric illness and substance abuse, this book discusses modeling of neuropsychiatric illness, drug abuse paradigms and techniques, biomarker identification, autoimmune inflammatory response and other topics.

This volume presents novel developments in UPS research and important methods related to recent advances in the field. The volume focuses on the enzymology and substrate identification of ubiquitin family modifiers, the recognition and chain formation of these modifiers, and more.

Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells.

Now recognized as a reservoir for growth factors and cytokines modulating cell activation status and turnover, proteoglycans have stimulated great amount of interest and research.

Including reproducible laboratory protocols, this guide to fungal pathogens in plants has been written by expert researchers in the field and includes methods now used to study them, including techniques for model systems such as Arabidopsis thaliana.

Characterization of copy number variation and other forms of structural genetic variation has highlighted the complexity of human genetic variation and also provided significant insight into the evolution and dynamic nature of our genome.

This volume presents an overview of the methods commonly used to study Mucins. These include the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics, in addition to detailed experimental protocols together with troubleshooting and interpretation tips.

These include methods and technique for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research.

The immediacy of individual genomes also serves to highlight our relative ignorance of human genetic variation, underlining the need for more studies of the nature and impact of genetic variation on human phenotypes.

Molecular profiling technology has advanced dramatically, particularly in the field of cancer tissue biomarkers. This title focuses on the entire process from discovery to commercialization. It helps scientists in understanding the advancements in genomics, proteomics, imagining, and bioinformatics.

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events¿changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely ¿embryology.¿ In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in ¿experimental embry- ogy¿ and the even more classical ¿chemical embryology,¿ the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the ¿cellular and molecular biology revolution.¿ The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Divided into convenient sections, this book contributes to directing the reader's attention to the many fields of growing scientific interest in non-natural amino acids. It includes methods and protocols, and provides step-by-step readily reproducible results.

This book describes the construction and purification of capsid-modified adenoviruses as well as oncolytic adenoviruses, presents protocols for many virus species, and describes the generation and testing of next-generation of oncolytic vaccinia virus.

This volume in the Methods in Molecular Biology(TM) series presents updated reviews of the chemistry of small molecules and their interaction with protein targets as well as detailed protocols that cover different types of ligands, carbohydrates, and lipids.

Carbohydrate microarrays emerged as a key technology for the deciphering of the glycospace. This book aims to give the reader the theoretical and experimental clues necessary for the fabrication and implementation of carbohydrate microarrays.

Display technologies are a powerful biological tool, yet the complexity of ribosome display methods has inhibited their use. This volume provides detailed and easily followed protocols with the aim of facilitating a greater uptake of these methods.

This Methods in Molecular Biology(TM) volume offers methods for applying the principles of nanotechnology to tissue regeneration. Covers delivery systems and biosensors, and techniques for studying interaction between cells, biomolecules and surfaces at nanoscale.

These methods and techniques include resources for microarray bioinformatics, microarray data analysis, microarray bioinformatics in systems biology, next generation sequencing data analysis, and emerging applications of microarray and next generation sequencing.

The rapid expansion of synthetic biology is due to the design and construction of synthetic gene networks that have opened many new avenues in fundamental and applied research.

New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength.

Coupling features of chromatography and mass spectrometry, SELDI is a popular method of biomarker discovery. This volume reviews its current applications, emphasizing study and experimental design, data analysis and interpretation, and assay development.

In the last decade the life sciences have evolved at a startling rate, culminating in today's high-throughput methods in biochemistry laboratories. In this book, expert researchers focus on dynamic aspects of the transcriptome, proteome and metabolome.

The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years.

In mTOR: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study mTOR. These include methods and techniques used for the study of the mTOR pathway and potential therapeutic applications of mTOR inhibitors such as, immunosuppressive and anticancer agents.

Recombinant gene expression research has brought new advances such as the birth of the 'omics' technologies. Here, expert researchers detail many RGE methods now used, including those for bacteria, fungi, plants and plant cells, and animals and animal cells.

This Methods in Molecular Biology(TM) book collects techniques applicable to the study of both the male and female germline. Includes lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting.