Bücher der Reihe Methods in Molecular Biology

"Recent studies have discovered new known and characterized cytokines, allowing for advancement in miniaturization of micro-analytical methods as well as the extensive development of bio-informatics and nanotechnology.

Divided into five convenient sections, Rho GTPase: Methods and Protocols provides an historical overview of the field and an account of the phylogenetics of the Rho family, general biochemical methods, and functional assays that allow monitoring the consequences of manipulating Rho GTPases in a variety of contexts.

Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs.

This Methods in Molecular Biology(TM) book is a comprehensive guide to techniques for astrocyte cell culture, and more specialized approaches for studying astrocyte functions, both in vitro and in vivo. Offers step-by-step protocols, troubleshooting tips and more.

The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping.

Due to the rapid and steady growth of available low-cost computer power, the use of computers for discovering and designing new drugs is becoming a central topic in modern molecular biology and medicinal chemistry.

In Liver Stem Cells: Methods and Protocols, expert researchers focus on several hepatic progenitor cells, hepatic differentiation form stem cells, bile ductal cell formation from stem cells, liver stem cells and hepatocarcinogenesi, and application of liver stem cells for cell therapy.

This book presents timely and important tools needed to elucidate particular roles of phosphoinositides in plants. It includes easy-to-use, reproducible protocols and contains expert tips on troubleshooting and avoiding known pitfalls.

This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. It features cutting-edge methods ready for the laboratory.

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states.

Structure-Based Drug Discovery presents an array of methods used to generate crystal structures of biological macromolecules, how to leverage the structural information to design novel ligands anew, and how to iteratively optimize hits and convert them to leads.

The 3rd edition of this Methods in Molecular Biology(TM) book offers detailed protocols and practical advice on modern approaches to the study of leucocytes and their products. Includes materials lists, step-by-step lab protocols, and key tips on troubleshooting.

This exploration of the culture and genetic manipulation of primary fungal pathogens, as well as interactions in model systems, includes detailed, lab-ready techniques as well as the key tips and implementation advice necessary to achieve successful results.

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

This Methods in Molecular Biology(TM) volume offers methods for investigating chromatin remodeling in vitro and in vivo, in yeast, plants and mammalian cells, and at local and global levels. Includes materials lists, protocols, troubleshooting tips and more.

The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago.

The variability of clinical symptoms in mitochondrial diseases calls for a systematic presentation of the full range of protocols used in their assessment. This volume provides cutting-edge methods of analysis as well as expert guidance on how to apply them.

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained.

In this volume expert researchers detail in silico methods widely used to study peptides. Chapters focus on integration and application of technologies to analyze, model, identify, predict, and design a wide variety of bioactive peptides, peptide analogues and peptide drugs, as well as peptide-based biomaterials.

This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction.

This volume provides an overview of RNA bioinformatics methodologies, including basic strategies to predict secondary and tertiary structures, and novel algorithms based on massive RNA sequencing.

This volume is essential for geneticists, molecular biologists, biochemists, and medical doctors interested in the use of mouse models in cancer research.

In this book, leading researchers in the field of Bioinformatics provide a selection of the most useful and widely applicable methods, able to be applied as is, or with minor variations, to many specific problems.

Since the first edition, published in 2001, genomics research has taken great strides. In this updated second edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products.

Volume I: Nuclei and Subnuclear Structures presents an overview of features of the intranuclear environment, followed by the most recent procedures for isolating nuclei from a wide range of cell types including muscle cells, yeast, oocytes, cells with polytene nuclei, Arabidopsis, trypanosomes, and dinoflagellates.

In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells.

Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome.

In Bioinformatics, leading researchers in the field provide a selection of the most useful and widely applicable methods, able to be applied as is, or with minor variations, to many specific problems. Over 80 authors from around the globe have contributed.

This, the second volume in the series, provides a comprehensive collection of the cutting-edge methods making a major contribution to understanding the nucleus and its nanostructure today. Chapters contain tips for avoiding known pitfalls.

This collection of robust, readily reproducible methods for microarray-based studies includes expert guidance in the optimal data analysis and informatics.