Bücher der Reihe Methods in Molecular Biology

This new edition provides updated and novel protocols of neuroproteomics methods that encompass both global-scale as well as targeted and specialized topics, which are timely additions for the molecular and phenotypic analysis of the central nervous system and CNS-related disorders.

This thorough volume provides an in-depth introduction to and discussion of microRNAs (miRs) and their targets, miR functions, and computational techniques applied in miR research, thus serving the need for a comprehensive book focusing on miR target genes, miR regulation mechanisms, miR functions performed in various human diseases, and miR databases/knowledgebases. Without prior knowledge of the area of study, computational biologists, computer scientists, bioinformaticians, bench biologists, as well as clinical investigators will find it easy to follow the techniques in this collection. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that ensures successful results. Accessible and practical, Bioinformatics in MicroRNA Research functions as an ideal guide for researchers of all backgrounds to explore this vital area of study.

This second edition provides up-to-date and new chapters covering the most important widely-used techniques in plant stress tolerance, including cutting-edge strategies, in a manner that ensures effective results. Plant Stress Tolerance: Methods and Protocols, Second Edition guides readers through reviews on epigenetics, long noncoding RNAs, and the influence of microbiome on drought tolerance, the volume then continues with methods describing the identification of stress-responsive genes, proteins, posttranslational redox modifications, DNA methylation, histone modifications, measurement of glutathione, lipids, raffinose family oligosaccharides, polyamines, as well as quantification of abscisic acid, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Plant Stress Tolerance: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

This volume provides practical experimental laboratory protocols for a wide range of steroid bioconversions. The chapters in this book cover topics such as bioconversions and chemical synthesis pathways; strain characterization; bioconversion from sterols to androstenedione and androstadienedione; steroid hydroxylations; biocatalysis; and downstream processes to purify steroid intermediates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Microbial Steroids: Methods and Protocols is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology.

This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution.

This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen studies.

This volume presents protocols for Brachypodium genomics in numerous areas ranging from marker development, trait evolution, functional genomics, metabolomics, transcriptomics, genomics, and tilling.

Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book.

Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes.

The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids.

This Methods in Molecular Biology(TM) book is written on the premise that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. Offers materials lists, protocols and troubleshooting tips.

Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge.

Out of the study of the intricacies of the genome and gene regulation, arose a new science that was independent of actual DNA changes, but critical in maintaining gene regulation and genetic stability.

The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins.

The tissue microarray (TMA) method presents as a modern high technology, although its roots go back to the 80s when researchers frst started to combine several small pieces of tissues into so-called sausage blocks.

This Methods in Molecular Biology(TM) book covers proteomics and mass spectrometry for studying protein modification, biochemical and cell biological tools for studying protein-protein interactions, genomic methods for dissecting protein-DNA interaction and more.

In this book, experts study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. It also covers related ethical and legal considerations.

This volume in Springer's Methods and Protocols series has expert researchers detailing many of the latest methods for studying canthaxanthin production, directed evolution of carotenoid synthases, and a host of other techniques, all with stepwise directions.

Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions.

The 2nd edition of this Methods in Molecular Biology(TM) volume provides novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Offers a toolkit enabling laboratories to maximize the power of this extraordinary experimental system.

Current knowledge of the mechanisms that regulate DNA repair has grown significantly over the past years with technology advances such as RNA interference, advanced proteomics and microscopy as well as high throughput screens.

This Methods in Molecular Biology(t) book details methods and and protocols used to study Helicobacter infection. Includes lists of materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and known pitfalls.

With new chapters covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques.

This Methods in Molecular Biology(TM) book offers methods for studying retinal homeostasis and disease, including cell culture systems and animal models of disease, their generation, phenotypic and molecular characterization and use in therapeutic approaches.

In addition, deficiencies of proteins that are engaged in assisting and supervising protein folding (protein quality control) may impair the folding of many other proteins, resulting in pathological phenotypes.

In this book, experts in proteomic research contribute detailed techniques which present an overview of the contemporary challenges and possibilities in the various areas of proteomics. The book makes a valuable contribution to proteomics research.

Neuroproteomics researchers, however, have pri- rily involved themselves dysfunction, based on a pressing need (and invariably funding), in answering questions on CNS dysfunction, based on a pressing need (and invariably funding), and because such questions hold more accessible answers.

In this new volume, renowned authors contribute fascinating, cutting-edge insights into microarray data analysis. Information on an array of topics is included in this innovative book including in-depth insights into presentations of genomic signal processing.