Bücher der Reihe Methods in Molecular Biology

Neuroproteomics researchers, however, have pri- rily involved themselves dysfunction, based on a pressing need (and invariably funding), in answering questions on CNS dysfunction, based on a pressing need (and invariably funding), and because such questions hold more accessible answers.

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host.

The advent of non-invasive imaging technology, such as magnetic resonance imaging (MRI), has allowed biologists and clinicians to make great strides in unraveling the secrets of the brain.

As the major task of sequencing the human genome is near completion and full complement of human genes are catalogued, attention will be focused on the ultimate goal: to understand the normal biological functions of these genes, and how alterations lead to disease states.

The discovery of Epstein-Barr virus (EBV) by Epstein, Achong, and Barr, reported in 1964 (Lancet 1:702-703), was stimulated by Denis Burkitt's rec- nition of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor's etiology (Nature194:232-234, 1962).

Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of applications.

In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism.

Here is a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for development and optimization of RNAi-based antiviral drugs. The methods here can also be applied outside the context of a virus infection.

This Methods in Molecular Biology(TM) book covers chemical probes of membrane protein structure and function, photochemical control of protein and cellular function, and chemical probes for imaging the nervous system. Offers step-by-step protocols, tips and more.

Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs.

Microelectronic engineering has revolutionized electronics. This book instills in the reader a working understanding of the methods underlying microengineering and the means by which such methods can be used for a range of analytical techniques.

In a single volume this book assembles the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. This is an essential resource for both experienced and novice researchers.

There have been remarkable advances in the study of human pathogenic fungi. In this authoritative book expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular.

Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro.

There have been remarkable advances in the study of human pathogenic fungi. In this authoritative book expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular.

Clinical epidemiology provides the scientific basis for the practice of medicine due to its focus on the diagnosis, prognosis and management of human disease using appropriate research design, measurement and evaluation.

This book sees expert researchers explore current issues and methodologies in this expanding field, addressing areas of gene discovery and functional analysis with a focus on the sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling.

Recent years have seen significant advances in technologies that enable us to analyze transcription factors, a pivotal cell regulatory process in plants. This comprehensive survey covers the basic concepts as well as detailed protocols of tools to study TFs.

In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology. Features key tips and advice, there is emphasis on mammalian cell culture systems.

It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, this book focuses on experimental approaches to better understand the complexity of signal transduction.

Written in the successful Methods in Molecular Biology (TM) series format, In Vivo NMR Imaging: Methods and Protocols aims to be an experimental compendium of modern in vivo MR imaging with special focus on recent developments in molecular imaging and new protocols for imaging metabolism and molecular markers.

This Methods in Molecular Biology(TM) book shows how PARP proteins act in normal and pathogenic development of organisms, advances knowledge of developmental pathways regulation, and furthers development of new drugs and methods to target PARP-dependent processes.

It is now more than ten years since Dr. Alec Jeffreys (now Professor Sir Alec Jeffreys, FRS) reported in Nature that the investigation of certain minisatellite regions in the human genome could produce what he termed DNA fingerprints and provide useful information in the fields of paternity testing and forensic analysis.

Putrescine and spermidine are ubiquitous in living organisms. Spermine, third of the three most commonly occurring natural polyamines, is probably present in all eukaryotes but is rare (or nonexistent) in prokaryotes. Polyamine residues are constituents of many compounds found in plants and insects. Putrescine, spermidine, or spermine-containing alkaloids are found in many plants, nonproteinaceous spider and wasp toxins contain polyamine residues, and glutathionyl¿spermidine conjugates have been found in some pathogenic microorganisms. In most cells polyamines are the products of a highly regulated bios- thetic pathway. It is not clear whether the elaborate regulation of polyamine synthesis is a consequence of their essential role(s) in cellular differentiation and development, or part of a defense mechanism to prevent overaccumulation of compounds that are toxic in excess. In addition to their biosynthetic capa­ bility, many cells also possess transport systems for polyamines that respond to intracellular polyamine levels, and other stimuli, and are regulated by mecha­ nisms that are at present incompletely defined. Two routes of polyamine catabolism have been identified in mammalian cells, a biodegradative route and a recycling pathway. The relative impor­ tance of these pathways and their overall regulation is only partially resolved. What is clear is the widespread occurrence of a variety of polyamine-oxid- ing enzymes in animals, plants, bacteria, and fungi. Polyamine catabolism, by whichever route, results in the formation of aminoaldehydes as intermediates.

General initiation factors are involved in positioning RNA polymerase II on the core promoter, but the complex - teraction of these proteins and transcriptional activators binding to DNA e- ments outside the core promoter regulate the rate of transcriptional initiation.

Neurodegeneration tackles the challenges involved with neurodegenerative disorders, such as Alzheimer's and Parkinson's disease. The text presents cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes.

Protein Expression in Mammalian Cells presents a compendium of vital techniques to further our knowledge of mammalian protein expression. Each chapter includes a brief introduction, step-by-step, readily reproducible laboratory protocols, and troubleshooting tips.

Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age.

Evaluating potential mutagenic activity is a critical step in assessing the safety of both new and pre-existing chemical types. In this book, expert contributors help to satisfy the demand for education in this vitally important field of research.

This selection of protocols and procedures includes the latest techniques and shows how to make use of cellular, tissue, and whole animal models which can then be applied to the investigation of neurotrophic factors and other agents impacting on these systems.