Bücher der Reihe Methods in Molecular Biology

Ribozymes details protocols and techniques commonly used to study individual ribozymes and their characterizations. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and troubleshooting tips.

This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.

In this book internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors and Design and Characterization of Gene Transfer Vectors.

This book sees expert researchers explore current issues and methodologies in this expanding field, addressing areas of gene discovery and functional analysis with a focus on the sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling.

In Gene Therapy Protocols, Volumes 1 and 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2).

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease.

Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement.

This Methods in Molecular Biology(t) book details methods used to study proteomics, offering lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

In Statistical Methods for Microarray Data Analysis: Methods and Protocols, expert researchers in the field detail many methods and techniques used to study microarrays, guiding the reader from microarray technology to statistical problems of specific multivariate data analysis.

The 2nd edition of this Methods in Molecular Biology(TM) book offers methods for investigating production and analysis of transgenic plants, focused on selection and detection methods, transformation technology, targeting, silencing and directed mutation and more.

As ever more petabytes of information are added to biological databases such as that for the human genome, this new volume in the Methods in Molecular Biology series explains how to convert the deluge of fresh data into new knowledge and biological insight.

This comprehensive new resource in Springer's Methods in Molecular Biology series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry.

Antigen processing is a biological process that prepares antigens for the presentation to special cells in the immune system called T lymphocytes.

It includes novel approaches and protocols that were not preservable when the first edition was published.The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation.

This current collection builds on this, but takes a broader view of proteome data analysis covering data analysis essentials, but also the databases and data models, as well as practical consid- ations for analysing database search results, annotating genomes, and speeding up searches.

Detailing current methodology, this book examines important analytical techniques in chiral separation sciences. It includes step-by-step, readily reproducible laboratory protocols and troubleshooting tips.

Lipid-Protein Interactions: Methods and Protocols provides a selection of protocols to examine protein-lipid interactions, membrane and membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer and lipid properties.

The study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In this book, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology.

This Methods in Molecular Biology(TM) book covers techniques for identifying protein acetylation, column- and gel electrophoresis-based approaches, computational prediction and biological response to protein acetylation. Includes protocols and troubleshooting tips.

This book offers a range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer. It features step-by-step detail essential for reproducible results and implementation advice from the experts.

This Methods in Molecular Biology(TM) book offers methods for molecular cytogenetics and chromosome analysis in plants. Includes state-of-the-art protocols for studying the organization and behavior of genetic material in a wide range of model and crop species.

Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive.

Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine.

Understanding an individual's genetic makeup is the key to creating personalized drugs with greater efficacy and safety, and pharmacogenomics aims to study the complex genetic basis of inter-patient variability in response to drug therapy.

Later chapters deal with statistical approaches to data analysis where the experimental objective is either to confirm the biology by identifying genomic regions associated to a trait or to use the data to make genomic predictions about a future phenotypic outcome (e.g.

This second edition of Nanobiotechnology Protocols expands upon the previous editions with current, detailed protocols for nanobiotechnology; imaging and detection. It includes new chapters that explore nano-bio constructs and toxicology of nanomaterials.

This book details various aspects of nanotechnology from the oxidative stress point of view, with a focus on synthesis of different antioxidant nanoparticles and antioxidant-loaded nanoparticles, as well as their in vitro/ in vivo mechanisms of action.

This Methods in Molecular Biology book offers methods for studying inflammasome function, including generation of inflammasome stimuli, monitoring of caspase-1 activity and processing, activation of IL-1 cytokines, plus lab protocols, material lists and tips.

Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book.

This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations.