Bücher der Reihe Methods in Molecular Biology

Protein phosphorylation controls many basic cellular processes, such as cell growth, differentiation, migration, metabolism, and cell death, and its study can provide key insights into the signal transduction pathways that are activated in cells in response to different stimuli, such as growth factor stimulation or exposure to toxicants.

This Methods in Molecular Biology(TM) volume offers detailed protocols for the preparation of plant genomic DNA, fingerprinting of plants for the detection of intra-species variations, use of DNA barcoding and methods for the bioinformatic analysis of data.

This Methods in Molecular Biology book reviews techniques for identifying bioactive compounds used in such areas of plant biology as pathogenesis, immune responses, small RNA processing, endomembrane trafficking, lipids, plant hormone signaling and cell wall.

In Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols, expert researchers in the field provide a complete overview of protocols used in the development of RNA molecule as drugs and drug target.

Human Fertility: Methods and Protocols is intended for all practitioners of reproductive medicine and ART, as well as for embryologists and reproductive, developmental, cell and molecular biologists and others in the biomedical sciences.

Researchers describe the major techniques necessary to study polyamines and their function. The techniques are presented with step-by-step instructions and numerous hints and tips to ensure reproducible results.

Over the last decade, interest in mycoplasmas has been greatly sti- lated by the spread of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp.

Stress Response: Methods and Protocols brings together a diverse array of practical methodologies that may be employed to address various aspects of the response of mammalian cells to environmental stress.

Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive.

In response to any of these assaults on the integrity of the genome, the activation of the network of checkpoint control pathways can lead to diverse cellular responses, such as cell cycle arrest, DNA repair, or elimination of the cell by cell death (apoptosis) if the damage cannot be repaired.

Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics.

While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side worker to identify a reliable experimental protocol in the ocean of the literature.

The methods described in this book do not cover all of the techniques currently used to study T cell-mediated immune responses for the simple reason that T cell immunology is probably the immunological discipline which can be investigated with the widest variety of approaches.

The tissue microarray (TMA) method presents as a modern high technology, although its roots go back to the 80s when researchers frst started to combine several small pieces of tissues into so-called sausage blocks.

The methods described in this book do not cover all of the techniques currently used to study T cell-mediated immune responses for the simple reason that T cell immunology is probably the immunological discipline which can be investigated with the widest variety of approaches.

With the huge potential of gene transfer into hematopoietic stem cells, active research in this field has become critically important. In this volume, leading scientists in the field provide a compendium of protocols which cover the subject comprehensively.

Protein phosphorylation controls many basic cellular processes, such as cell growth, differentiation, migration, metabolism, and cell death, and its study can provide key insights into the signal transduction pathways that are activated in cells in response to different stimuli, such as growth factor stimulation or exposure to toxicants.

This volume, SUMO Protocols, therefore aims at presenting a collection of methods relevant to SUMO research in order to make these tools available to biochemists, molecular and cell biologists as well as research-oriented clinicians not yet familiar with the system.

Bacterial Molecular Networks provides authoritative descriptions of various experimental and computational methods that enable the characterization and analysis of molecular interaction networks.

Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells.

Microelectronic engineering has revolutionized electronics. This book instills in the reader a working understanding of the methods underlying microengineering and the means by which such methods can be used for a range of analytical techniques.

In this volume, leaders in the field share a diversity of cutting-edge techniques in endocrinology. Coverage includes protocols that allow investigators at all stages of their scientific career to successfully perform these techniques.

Exciting new biological functions are still being discovered for vitamin A derivatives, including the myriad physiological activities of retinoids. Here, experts in the field present the latest range of techniques for both in-vitro and in-vivo studies.

As a fairly new and expansive area of study, plant systems biology has been approached by scientists from fields as varied as plant physiology and astrophysics, creating a wide variety of techniques and methods to further this vital research.

This Methods in Molecular Biology(TM) book reviews methods and techniques used for exon skipping, such as methods on how to distinguish "real polymorphisms" from mutations that affect splicing. Includes protocols, materials lists, pitfalls and troubleshooting tips.

Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes.

The last 10 years have witnessed an explosion in our understanding of plant h- mones. The greatest strides in plant hormone biology have come, by and large, from the use of genetic methods to identify receptors and we dedicate a chapter to general genetic methods of analysis using the model system Arabidopsis thaliana.

Since the discovery of DNA structure and throughout the ensuing "DNA era", the field of DNA replication has expanded to cover a vast number of experimental systems.

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease.