Bücher der Reihe Methods in Molecular Medicine

Viral Vectors for Gene Therapy: Methods and Protocols consists of 30 ch- ters detailing the use of herpes viruses, adenoviruses, adeno-associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems for vector development and gene transfer.

Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines.

Leading experts in the use of MRI explain its basic principles and demonstrate its power to understand biological processes with numerous cutting-edge applications.

This book provides a comprehensive volume that integrates a wide spectrum of methods required to implement current and future progress in tissue engineering. The book supplies an understanding of diverse technologies and methods used to drive tissue engineering into a clinical reality.

Here is a compendium of data pertinent to the methods and protocols that have contributed to both recent advances in molecular medicine in general as well as to molecular basis of rheumatic disease in particular.

This volume is a compendium of cutting-edge molecular methods for the successful transplantation of hematopoietic stem cells. They describe promising tools for stem cell transplant research models, such as in vivo bioluminescence imaging. They discuss HLA typing, PCR-SSP typing, and HLA antigens.

Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it.

Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies.

This is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. Leaders in the field contribute to cover exciting and cutting edge topics.

Blood-brain barrier (BBB) breakdown leading to cerebral edema occurs in many brain diseases-such as trauma, stroke, inflammation, infection, and tumors-and is an important factor in the mortality arising from these con- tions.

During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess.

Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical manifestations of malaria and the Plasmodium parasite, there is still no licensed vaccine for the prevention of malaria.

Despite a growing awareness of OA as a medical problem that has yet to reach its maximum impact on society, there is a surprising absence of effective medical treatments beyond pain control and surgery.

A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology.

Hemoglobin and Hemoglobinologists This volume, Hemoglobin Disorders: Molecular Methods and Protocols, will be introduced with a review of the great milestones in the field, and the scientists responsible for those achievements.

We are currently experiencing a fundamental shift in the way in which we approach the characterization of cancer. These drugs are only the forerunners of a coming tidal wave of novel therapeutics that individually target specific m- ecules within cancer cells-more than 300 such agents are currently in phase I or II clinical trials.

Despite the availability of an effective vaccine, there are still 400 million people, worldwide who are chronically infected with hepatitis B virus (HBV). Given the possible consequences of HBV infection, the number of those chronically infected with HBV presents an enormous public health challenge.

Opioid research is one of the multidisciplinary research areas that involve advanced techniques ranging from molecular genetics to neuropharmacology, and from behavioral neuroscience to clinical medicine.

A collection of basic and advanced molecular methods that reveal those markers essential for diagnoses of specific diseases. The techniques range from in vitro methods to in vivo models of prostate cancer, and include new methods for the accurate diagnosis of prostate cancer, and proteome and microarray analyses.

Suicide Gene Therapy: Methods and Reviews covers gene therapy that targets malignant cells in a treatment that has become known as "suicide gene therapy. Additional trials used multiple gene therapy protocols that also involved suicide gene therapy (83 with 497 patients), indicating that the interest in this area is considerable.

We are in an exciting era in the war against cancer, with real prospects for novel anticancer drugs that are cancer cell-specific without the toxicities that have been the hallmark of conventional cytotoxic cancer chemotherapy.

Describes a variety of laboratory methods being used for investigating and treating hepatocellular carcinoma. This book focuses on gene therapy approaches, including the use of such vectors as lipids, such viruses as adenovirus and baculovirus, and virus detection assessment using electron microscopy.

Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines.

The aim of Alzheimer's Disease: Methods and Protocols is to bring together the main biochemical, cell biological, and molecular biological techniques and approaches that are being used to investigate the molecular basis of Alzheimer's disease.

James Gray and Ulrich Desselberger have assembled a comprehensive collection of established and cutting-edge methods for studying and illuminating the structure, molecular biology, pathogenesis, epidemiology, and prevention in animal models of infection with rotaviruses, an important cause of infant morbidity and mortality.

Septic shock remains a serious medical condition with high mortality. Septic Shock Methods and Protocols is an attempt to draw together into one volume a number of protocols that are of use in the investigation of the mechanisms of septic shock.

The purpose of this volume of Methods in Molecular Medicine is to set forth examples of the great variety of techniques and applications that are now emerging in the field of nonviral gene therapy.

Presents a collection of the techniques for studying the biology and immunopathology of celiac disease. This title contains detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning.

There is now a range of cytotoxic drugs that have considerable clinical usefulness in producing responses in tumors and even, in a small proportion of cases, cure.