Bücher der Reihe Principles and Practice

Provides a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. This book presents the methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS).

Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.

Several representative hemato-oncologic malignancies are analyzed in detail: acute lymphoblastic leukemia, acute myeloid leukemia, B-cell Non-Hodgkin lymphomas, multiple myeloma, chronic lymphocytic leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and myeloproliferative neoplasms.

This book offers a comprehensive selection of essays by leading experts, which covers all aspects of modern imaging, from its application and up-scaling to its development. There is ample practical and detailed "how-to" content on important, but rarely addressed topics.

This book offers a comprehensive selection of essays by leading experts, which covers all aspects of modern imaging, from its application and up-scaling to its development. There is ample practical and detailed "how-to" content on important, but rarely addressed topics.

'Methods in Proteome and Protein Analysis' presents the most interesting papers from the 14th MPSA meeting, including those on mass spectrometry for protein research, protein-protein interaction using MALDI-MS, and the classification and prediction of structure or functional sites.

In spite of tremendous scientific progress over the past years, cell biologists do not yet understand the fundamental processes that determine the life cy cle of a cell. Such are: cell movement and cell spreading, cell division, cell communication, cell signaling, cell regeneration and cell death.

Paul Popescu, Directeur de Recherche at INRA, has also played a promi nent part in the development of animal cytogenetics, especially in domestic animals. She showed the fascinating correspondence between bovine and human chromosomes and the com pared gene maps of domestic bovidae.

This volume describes high-throughput approaches to a series of robust, established methodologies in molecular genetic studies of population samples.

Recent advances in large scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Mass spectrometry is the method of choice for the rapid large-scale identification of these proteomes and their modifications.

With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research.

Several representative hemato-oncologic malignancies are analyzed in detail: acute lymphoblastic leukemia, acute myeloid leukemia, B-cell Non-Hodgkin lymphomas, multiple myeloma, chronic lymphocytic leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and myeloproliferative neoplasms.

This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols.

This volume describes high-throughput approaches to a series of robust, established methodologies in molecular genetic studies of population samples.

This book provides a clear conceptual description of each facet of proteomics, describes recent advances in technology and thinking in each area, and provides details of how these have been applied to a variety of biological problems.

The book provides an updated overview of molecular analysis of human tissues, and the impact this analysis has on diagnosis and prognosis of human diseases.

Paul Popescu, Directeur de Recherche at INRA, has also played a promi nent part in the development of animal cytogenetics, especially in domestic animals. She showed the fascinating correspondence between bovine and human chromosomes and the com pared gene maps of domestic bovidae.

The book provides an updated overview of molecular analysis of human tissues, and the impact this analysis has on diagnosis and prognosis of human diseases.

This book provides a clear conceptual description of each facet of proteomics, describes recent advances in technology and thinking in each area, and provides details of how these have been applied to a variety of biological problems.