Bücher veröffentlicht von Humana Press Inc.

Leading gene therapy researchers and clinicians illuminate the field-from basic vector technology to current and future clinical applications in neurology.

Strategies of treatment involving therapeutic proteins, irnrnune immune cells, or cel lular protein targets are those of greatest potential for further reducing mortality from melanoma.

The text concludes with appendices that provide educational tools for symptom monitoring and dietary guidance.Written by specialists in their respective fields, Celiac Disease is a comprehensive resource that provides the healthcare practitioner an evidence-based practical guide to pathogenesis and therapy of celiac disease.

Key workers in vascular medicine and biology apply state-of-the-art techniques in cell and molecular biology to study the endothelial and vascular smooth muscle cells.

During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess.

Providing step-by-step instructions to assure successful experimental results, this title covers the key methods for mutation detection and screening, including discussions of quantitative PCR, trinucleotide repeat detection, sequence-based mutation detection, fluorescence in situ hybridization (FISH), and in vitro protein expression systems.

Cardiac Cell and Gene Transfer: Principles, Protocols, and Appli- tions addresses principles and applications of cell and gene transfer to the heart, including protocols for vector production and purification.

Thus, a proper understanding of the inflammatory basis may provide clues to new therap- tic targets not only in classical inflammatory diseases, but atherosclerosis, cancer, and ischemic heart disease as well.

Describes the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis. This book presents a set of enzymatic methods for determining DNA breaks.

Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors. We are confident that Cell Cycle Control and Dysregulation Protocols will facilitate and optimize the work of practical scientists involved in researching the cell cycle.

The rapid growth in the number of options available for the management of colorectal cancer presents the clinician with new opportunities and new complexities.

From the viewpoint of this volume, lung inflammation is now recognized as central to the pathophysiology of a number of severe respiratory conditions, the two most common being asthma and chronic obstructive pulmonary disease (COPD).

There are several chapters devoted to the microorganisms associated with fermented foods: these are of increasing importance in food microbiology, and include one bacteriophage that kills the lactic acid bacteria involved in the manufacture of different foods-cottage cheese, yogurt, sauerkraut, and many others.

Available separately or in a three volume set, this work is a highly practical laboratory manual that should be of great use to students and teachers of developmental biology at all levels.

In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting;

Numerous cancer gene therapy strategies are currently being investigated, including gene replacement therapy, the regulation of gene expression to modulate immu- logical responses to tumors, the direct killing of tumor cells, and direct int- ference with tumor growth.

In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio.

Although urology is a surgical specialty, it has become apparent that changes in health care delivery and financing have led to an increasing volume of care being provided by urologists in their offices.

The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations.

The last two decades have seen prodigious growth in the application of brain imaging methods to questions of substance abuse and addiction. Many more investigations of drug effects on the structure and function of the human brain were soon to follow, leading to much better insights into brain systems.

Describes in step-by-step detail the major molecular, biochemical, and functional techniques used in probing the renin-angiotensin system. This work includes techniques that range from the use of embryonic gene targeting to create of mutant strains, to the functional investigation of components of the renin-angiotensin system.

The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures to utilize this new resource for analysis of genetic variation and for the detection of disease causing mutations.

Parkinson's disease is a progressive neurodegenerative disorder charact- ized clinically by tremor, rigidity, slow movements, and postural instability. The chronic disability and the increased prevalence of the disease with the prolongation of life expectancy in developed countries make the social and economic impact of this disease quite high.

Further mouse genetic studies, involving animals carrying balanced translocations (reviewed in Chapter 3), indicated that imprinted genes were likely to be widely scattered and would form a minority within the mammalian genome.

The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

The observation that neuropeptide Y (NPY) is the most abundant peptide present in the mammalian nervous system and the finding that it elicits the most powerful orexigenic signal have led to active investigations of the properties of the NPY family of hormones, including peptide YY (PYY) and pancreatic polypeptide (PP).

In its broadest meaning, pathology is the study of disease, and therefore it follows that any disease for which the molecular basis is understood would be suitable as a topic for inclusion in this work.

More important, connexins have gained visibility because mutations in some connexin genes have been found to be linked to human genetic disorders. Connexin Methods and Protocols presents in detail a collection of te- niques currently used to study the cellular and molecular biology of connexins and their physiological properties.