Bücher veröffentlicht von Humana Press Inc.

This volume on Asthma: Mechanisms and Protocols in the Methods in Molecular Medicineseries provides an overview of the molecular mechanisms involved in asthma by p- viding extensive protocols that are being used in asthma research.

Over the past 20 years, technological advances in molecular biology have proven invaluable to the understanding of the pathogenesis of human cancer. The aim of Molecular Ana- sis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular changes in human cancer.

Stress Response: Methods and Protocols brings together a diverse array of practical methodologies that may be employed to address various aspects of the response of mammalian cells to environmental stress.

Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells provides review and protocol chapters that completely cover this relatively new scientific discipline.

This text focuses on the clinically important trace elements and minerals. These nutrients have a major impact on health and disease. The book reviews conditions (such as pregnancy) and major diseases that are influenced by trace element and mineral nutrition.

A collection of reviews which comprehensively examines the status of novel antifolates, an important class of anticancer drugs. This book discusses the preclinical and clinical pharmacology of methotrexate, other dihydrofolate reductase inhibitors, 5-fluorouracil, and a new generation of antifolates.

The aim of Ovarian Cancer: Methods and Protocols is to provide for ovarian cancer researchers in the first instance, a laboratory handbook that will facilitate research into cancer systems by providing a series of expert protocols, with proven efficacy, across a broad range of technical expertise.

A three-volume collection of protocols that presents reproducible methods for studying and analyzing the events of embryonic development.

This latest addition to the Methods in Molecular Medicine series, Anti- ral Methods and Protocols, is opportune because there is an increasing int- est in discovering compounds that are effective against both chronic and acute viral infections.

A three-volume collection of protocols that presents reproducible methods for studying and analyzing the events of embryonic development.

Over the last decade, interest in mycoplasmas has been greatly sti- lated by the spread of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp.

The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.

A major success story of modem molecular biology is the development of technologies to clone and express specific genes. Current applications of recombinant gene products cover a wide spectrum, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, agriculture and animal husbandry, and so on.

Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of "expression constructs" and their introduction into appropriate host cells and/or organisms.

The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms.

Researchers describe the major techniques necessary to study polyamines and their function. The techniques are presented with step-by-step instructions and numerous hints and tips to ensure reproducible results.

While working with these libraries, we noticed the interesting fact that when combinatorial libraries of oligopeptides were allowed to interact with different selector proteins, only the actual binding sites of these proteins showed binding properties, whereas the rest of the p- tein surface seemed "inert.

This work reviews the progress that has been made in strategies for testing possible mechanisms for neuronal cell death resulting in an understanding of the molecular epidemiology of dementia.

The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.

Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of "expression constructs" and their introduction into appropriate host cells and/or organisms.

The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique.

Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves tigation of gene function and dysfunction.

Since the initial description of techniques to immortalize anti body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species.

Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves tigation of gene function and dysfunction.

This arrangement is based on what are ostensibly increasing levels of integrated activity of the nervous system and, therefore, increasing levels of behavioral integration.

Similar reasons exist for Volume 4 on receptor binding techniques, since experimental details are provided for all types of ligand-receptor binding, including chapters on general principles, drug discovery and development, and a most useful appendix on computer programs for Scatchard, nonlinear, and competitive d- placement analyses.

In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology.

Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions.