Bücher veröffentlicht von Humana Press Inc.

Leading practitioners describe in detail advanced methods of mass spectrometry used in structural characterization of biomacromolecules of both natural and recombinant origin.

Alcohol and other drugs of abuse cause significant physi ological changes, especially during development. Clearly, understanding of the role women must take in modifying their alcohol and drug use during pregnancy will facilitate changes in our cultural and educational practices that will help reduce fetal trauma from alcohol.

This Methods in Molecular Biology(TM) book offers methods for molecular cytogenetics and chromosome analysis in plants. Includes state-of-the-art protocols for studying the organization and behavior of genetic material in a wide range of model and crop species.

This Methods in Molecular Biology(t) book details methods which are now commonly used to study skin stem cells. These include protocols, materials lists, tips and techniques for the isolation, maintenance and characterization of stem cell populations from skin.

This Methods in Molecular Biology(TM) book offers protocols for glycoproteins and glyco-engineered biopharmaceuticals with a focus on mAbs. Covers glyco-engineering of therapeutic proteins, glycoanalytics, glycoprotein complex characterization and PK/PD assays.

While many cytokines are known for their inflammatory action, there is a growing interest in the tissue-protective effects of some cytokines.

The prevalence of hypertension is almost three times as high as that of diabetes mellitus type 2, with both conditions being major risk factors for stroke, ischemic heart disease, cardiac arrhythmias, and heart failure.

This is the first book to examine organelle proteomics in depth. It begins by introducing the different analytical strategies developed and successfully utilized to study organelle proteomes, and detailing the use of multidimensional liquid chromatography coupled to tandem mass spectrometry for peptide sample analysis.

Diagnosis and Treatment of Human Mycoses brings together globally-recognized mycoses experts to guide readers in the use of current knowledge in the field of medical mycology to manage those who suffer from fungal infections (mycoses).

In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism.

This volume focuses on detecting different cellular stresses, measuring pathological consequences within the cell, and investigating the role of cellular stresses in select diseases. In addition, this book reviews the crosstalk between different stress pathways, stress responses during ageing, and targeting stress for regenerative medicine.

The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.

Chronic liver failure is a frequent condition in clinical practice that encompasses all manifestations of patients with end-stage liver diseases. Chronic liver failure is a multiorgan syndrome that affects the liver, kidneys, brain, heart, lungs, adrenal glands, and vascular, coagulation, and immune systems.

Here, an array of talented researchers covers our current state of knowledge in this exciting field. Four sections cover applications of stem cell techniques to mechanical regeneration, electrical regeneration, cardiac tissues and in vivo stem cell therapies.

Hematopathology: Genomic Mechanisms of Neoplastic Diseases will keep physicians abreast of the rapid and complex changes in genomic medicine, as exemplified by the molecular pathology of hematologic malignancies.

Endoscopic ultrasound has revolutionized the approach to lesions inside and outside the gastrointestinal tract.

Protein phosphorylation controls many basic cellular processes, such as cell growth, differentiation, migration, metabolism, and cell death, and its study can provide key insights into the signal transduction pathways that are activated in cells in response to different stimuli, such as growth factor stimulation or exposure to toxicants.

Molecular Diagnostics: 12 Tests That Changed Everything focuses on specific laboratory tests and emphasizes how the availability of these tests has altered how clinicians treat their patients.

Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs ('de-orphanizing' CYP substrates).

Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine.

This Methods in Molecular Biology(t) book details current procedures for identifying and validating new drug targets. Includes methods and approaches covering biochemical, cell based, in vivo models and translational methods, as well as relevant case reports.

This book offers a range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer. It features step-by-step detail essential for reproducible results and implementation advice from the experts.

In Rat Genomics: Methods and Protocols, world-wide experts provide both practical information for researchers involved in genomic research in the rat along with a more contextual discussion about the usefulness of the rat in physiological or translational research in different organs and systems.

Within the last 10 years, it has also become apparent that the familiar DNA duplex is not the only structure that can be targeted by DNA-binding ligands and there has been increased interest in triplex and quadruplex structures as drug targets, as well as protein- DNA complexes, such as those with nucleosomes or topoisomerases.

The last 10 years have witnessed an explosion in our understanding of plant h- mones. The greatest strides in plant hormone biology have come, by and large, from the use of genetic methods to identify receptors and we dedicate a chapter to general genetic methods of analysis using the model system Arabidopsis thaliana.

Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. This book describes the technologies in extensive technical detail, allowing readers to understand the technology and to construct similar devices.

This book reviews novel approaches developed to reverse tumor cell resistance to chemo/immuno/radio-therapy and the use of various sensitizing agents in combination with various cytotoxics. It also introduces several current approaches developed by established investigators that are aimed at overcoming resistance.

One out of seven newly diagnosed malignancies worldwide result from infection and chronic inflammation in conjunction with cancer. This book is a guide for fighting the 2,200 inflammation-related oncogenesis fatalities that occur every day.

Asian Americans are the fastest growing minority group in the United States. In this book, distinguished researchers and clinicians discuss the process of acculturation for individuals and their families, addressing the mental health needs of Asian Americans.

This volume outlines and examines current understanding of botulinum toxin and its various therapeutic applications. Written by leaders in their fields respected for their progressive approach to treatment, the book encourages responsible research into new and novel uses of botulinum toxin.