Bücher veröffentlicht von Humana Press Inc.

Studies on stem cells have been attracting intense scientific and p- lic attention, not only because of controversies surrounding the use of embryonic stem cells but also because of very provocative data that have been emerging on adult stem cells.

This Methods in Molecular Biology(TM) book covers techniques for identifying protein acetylation, column- and gel electrophoresis-based approaches, computational prediction and biological response to protein acetylation. Includes protocols and troubleshooting tips.

The book offers an introduction to the amoebozoa, presents large-scale analysis methods made possible by the completion of the Dictyostelium genome sequence, and details molecular genetics techniques, cell biological, biochemical and biophysical methods.

Capillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications.

This book provides guidance to those wishing to create enzyme variants. It covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes and the engineering of two difference types of rare-cutting endonucleases.

This volume offers cutting-edge technologies for studies of gene regulation. The chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory protocols.

This title offers procedures to investigate the interaction of membrane proteins, methods to study the biogenesis of membrane proteins and the dynamics of organelles, and protocols to analyze the functions or complex organization of membrane proteins.

Embryonic Stem Cell Immunobiology: Methods and Protocols covers a variety of relevant topics, such as hematopoietic stem cells derived from ES cells, the interaction of these cells with natural killer cells or with cytotoxic T cells, and specific protocols for the derivation of hematopoietic cells and neuronal cells, to name a few.

The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense;

This book details various aspects of nanotechnology from the oxidative stress point of view, with a focus on synthesis of different antioxidant nanoparticles and antioxidant-loaded nanoparticles, as well as their in vitro/ in vivo mechanisms of action.

In the diagnosis and treatment of movement disorders, the use of neuroimaging has expanded widely and has been an exciting, important modality for unlocking the causes of abnormal motor control.

This second edition of Nanobiotechnology Protocols expands upon the previous editions with current, detailed protocols for nanobiotechnology; imaging and detection. It includes new chapters that explore nano-bio constructs and toxicology of nanomaterials.

Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces.

Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease.

This Methods in Molecular Biology(TM) book offers methods to identify elements of the NO/cGMP/PKG pathway and to characterise function, signaling, localisation and importance on a cellular level and in whole animal models. Includes trouble-shooting tips and more.

Rising occurrences of various diseases and epidemics have pressurized the already-burdened health system across the globe, and this imposes an unprecedented challenge on our current research in identifying disease-specific biomarkers and molecular targets, in particular for cancers, neurological disorders and unexplained infertility.

Later chapters deal with statistical approaches to data analysis where the experimental objective is either to confirm the biology by identifying genomic regions associated to a trait or to use the data to make genomic predictions about a future phenotypic outcome (e.g.

This book details protocols that will help facilitate future research in developmental neuroscience. Coverage includes techniques of culturing neurons and glia, methods of gene delivery and down regulation, and protocols for analyzing axon growth.

This book details methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states. It features step-by-step, readily reproducible laboratory protocols as well as lists of the necessary materials and reagents.

Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine.

Understanding an individual's genetic makeup is the key to creating personalized drugs with greater efficacy and safety, and pharmacogenomics aims to study the complex genetic basis of inter-patient variability in response to drug therapy.

In-depth chapters review the potential long term consequences of menopause on the overall health of women, not only at the physical level including hot flushes (flashes) , alterations to the genitourinary system, skin changes, decreased cardiovascular functions, hypertension, headache, back pain, and constipation.

This book reviews the history of PPAR discovery and details receptor structure and its posttranslational modifications, discusses endogenous and exogenous ligands, the tissue distribution and versatile functions of PPAR subtypes in major organs and much more.

While specialists often guide the care to lung cancer patients, it is often a general radiologist who is left to interpret studies that impact patient care and management.

Skin Cancer: A Practical Approach presents a comprehensive and up to date overview on skin cancer.