Bücher veröffentlicht von Humana Press Inc.

This Methods in Molecular Biology(TM) volume offers methodologies enabling researchers to interrogate every aspect of mammalian oocyte development including recombination, meiotic maturation, oocyte substrate uptake, chromosomal segregation and fertilisation.

The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins.

Since its first application, microdialysis has become incredibly popular to study brain function and has been applied with success in different fields from psychopharmacology, neurobiology, and physiology in animals and also humans.

The mature T and NK cell lymphomas are rare, comprising approximately 10% of all malignant lymphomas. While the overall incidence of B-cell lymphomas has begun to decline in the United States, the incidence of T-cell lymphomas continues to rise.

A Clinical Guide to Occupational and Environmental Lung Diseases delivers a concise compendium to the diagnosis and management of occupational and environmental lung diseases, incorporating evidence-based guidelines where available.

In Diabetes and Peripheral Vascular Disease, a panel of distinguished leaders in the field of medicine, podiatry, and vascular and endovascular therapy assimilate the latest literature on these issues and others for an in-depth review of the management of peripheral vascular disease.

This comprehensive new resource in Springer's Methods in Molecular Biology series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry.

Cardiac tumors were once a nosographic entity of scarce clinical interest because of the rarity and of the intrinsic diagnostic and therapeutic impossibilities, and were considered a fatal morbid entity.

Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way.

This volume in the Methods in Molecular Biology(TM) series explores light microscopy techniques; electron microscopy; scanning probe techniques and complementary methods including soft x-ray tomography and secondary ion mass spectrometry.

The various international regulatory requirements are explained in detail, in order that the reader may perform all of the necessary studies for the successful registration or marketing authorisation of a new pharmaceutical, industrial chemical, crop protection product or food additive.

This Methods in Molecular Biology(TM) book covers microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methods from basic fabrication methods to commercializing research. Includes materials, protocols, tips and more.

With each chapter written by expert specialists, this publication reflects the key recent advances in molecular pathology that are contributing to the development of pediatric oncology. The book is structured according to organ systems for ease of reference.

Another key addition to Springer's Methods in Molecular Biology series, this volume includes methods for direct biolistic transfection of DNA using ballistic devices such as a gene gun. Lab-ready protocols and expert troubleshooting guarantee reproducibility.

The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule.

In the past decade there have been many research advances, such as directed differentiation of embryonic stem cells and induced pluripotent stem cells, robotic high throughput screening, whole genome siRNA and shRNA libraries, massively parallel sequencing at low cost, identification of somatic stem cells in key organs, to name a few.

This Methods in Molecular Biology(TM) book gathers tools for studying protein structure and function and enzymatic activities, purifying and analyzing of macromolecules and their complexes and investigating regulatory mechanisms and cell biological processes.

Detailing current methodology, this book examines important analytical techniques in chiral separation sciences. It includes step-by-step, readily reproducible laboratory protocols and troubleshooting tips.

Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers.

This Methods in Molecular Biology(TM) book covers mRNA synthesis, modifications and delivery, offering protocols, material lists and practical tips along with examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research.

The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

The traditional approach of applying tried-and-true solutions to speci?c pollution problems has been a major contributing factor to the success of environmental en- neering, and has accounted in large measure for the establishment of a "methodology of pollution control.

Studies are numerous and incorporate data on stroke inheritance, chromosomal loci of risk, preclinical models of stroke, and differential gene expression of brain injury, repair, and recovery.

A critical review of the classic, as wells as most recent-and quite seminal-findings concerning the phenotypic and molecular characteristics of both fetal and neonatal B and T cells, the cells that mediate antibody and cellular immune responses in newborns and infants.

In part through cross-species compa- sons and multidisciplinary approaches (combining, for example, classical embry- ogy, genetics, molecular biology, and systems biology), major questions have often been redefined and examined from new angles and with innovative tools.

Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria.

Protein Arrays: Methods and Protocols is an introduction to protein array technology and its application to the multiplexed detection of proteins. Although protein array technology has some roots in gene array technology, it can only be described as a distant relative.

In the literature, several terms are used synonymously to name the topic of this book: chem-, chemi-, or chemo-informatics. A widely recognized de- nition of this discipline is the one by Frank Brown from 1998 (1) who defined chemoinformatics as the combination of "all the information resources that a scientist needs to optimize the properties of a ligand to become a drug. " In Brown's definition, two aspects play a fundamentally important role: de- sion support by computational means and drug discovery, which distinguishes it from the term "chemical informatics" that was introduced at least ten years earlier and described as the application of information technology to ch- istry (not with a specific focus on drug discovery). In addition, there is of course "chemometrics," which is generally understood as the application of statistical methods to chemical data and the derivation of relevant statistical models and descriptors (2). The pharmaceutical focus of many developments and efforts in this area-and the current popularity of gene-to-drug or si- lar paradigms-is further reflected by the recent introduction of such terms as "discovery informatics" (3), which takes into account that gaining kno- edge from chemical data alone is not sufficient to be ultimately successful in drug discovery. Such insights are well in accord with other views that the boundaries between bio- and chemoinformatics are fluid and that these d- ciplines should be closely combined or merged to significantly impact b- technology or pharmaceutical research (4).

The authors delineate an up-to-date picture of platelet biology and describe methods for assessing platelet function, including the commonly used platelet aggregation, thromboxane production, procoagulant function, platelet function under flow, and the expression of platelet activation markers.

Expert clinicians comprehensively review the endocrine and metabolic responses to critical illness, explore the mechanisms and outcomes (positive and negative) of those responses to severe stress, and consider possible endocrine interactions that are not yet fully defined.