Bücher veröffentlicht von Humana Press Inc.

Divided into convenient sections, this book contributes to directing the reader's attention to the many fields of growing scientific interest in non-natural amino acids. It includes methods and protocols, and provides step-by-step readily reproducible results.

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events¿changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely ¿embryology.¿ In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in ¿experimental embry- ogy¿ and the even more classical ¿chemical embryology,¿ the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the ¿cellular and molecular biology revolution.¿ The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Soon after the first description of monoclonal antibodies in 1976, there was enormous interest in the clinical application of antibodies, especially in the context of cancer.

These essays are not simply a collection, but were developed from a single conception of the four ethical concerns of trans plan tation described in the first chapter. We have made room for, and indeed have invited, different and sometimes conflicting points of view on the complicated ethical ques- tions raised by transplant operations.

This multidisciplinary volume highlights novel nuclear analytical techniques and their applications to the life sciences. The methods and approaches presented are treated in a comprehensive and coherent manner suited to the needs of today's biological and bioanalytical researchers.

A two-volume guide to the purification of most receptor types, including receptors for neurotransmitters, polypeptide hormones, steroid hormones, and a variety of other ligands involved in many biological processes. It provides a comprehensive source of techniques to enable the purification of cloned receptors.

The purpose of this book-the fourth volume of a series on Can cer Markers-is intended to provide an updated "status report" on today's use of cancer markers in the diagnosis and monitoring of can cer, with an emphasis on cancer markers detected in the serum.

The Epstein-Barr virus (EBV), isolated in 1966, continues to draw worldwide attention as an important human pathogen. The second international symposium was held in St. Petersburg, Florida, in 1986, and was attended by approximately twice as many partici pants as attended the Loutraki meeting.

The role of carcinogenic agents in the deveolopment of human cancers is now being defined using a variety of human cells as experi- mental model systems.

This third volume of Drug and Alcohol Abuse Reviews focuses on the strategies currently thought best for the treatment of alcohol and tobacco abuse. A variety of approaches to treating alcohol abuse employ those psychosocial factors that are known to influence alcohol use in youth and adults.

Much of our knowledge of the cerebral circulation has been derived from studies of species other than human. " The authority of Galen's writings dominated scientific thought for about 1500 years.

This challenge is particularly difficult to meet in osteoporosis, not only because has been so much new knowledge generated in recent years, but also because this there disorder has not caught the attention of many practicing physicians.

Adenosine 3 ,5 -cyclic monophosphate (cAMP) and guanosine 3 ,5 -cyclic monophosphate are ubiquitous nucleotides that have been described as the first and second messengers. In concert with intracellular calcium and IP3, they play a major role in the control of intracellular signaling, which orchestrates normal and pathophysiological responses. Downstream from the cyclic nucleotide synthesis by adenylyl and guanylyl cyclases, the multigenic family of cyclic nucleotide phosphodiesterases (PDEs), by specifically hydrolyzing cyclic nucleotides, controls cAMP and cGMP levels to maintain a basal state. Their critical role in intracellular sign- ing has recently designated them as new therapeutical targets. Several leading pharmaceutical companies are searching and developing new therapeutic agents that would potently and selectively inhibit PDE isozymes, notably PDE4 and PDE5. Nevertheless, the precise mechanism and the contribution of the various PDE isozymes in modulating intracellular signaling remain to be established. The aim of Phosphodiesterase Methods and Protocols is to provide a palette of a variety of conceptual and technical approaches designed to solve qu- tions concerning the role of PDEs, and ultimately of their different variants, in physiological functions as well as their implications in several pathologies.

In the areas of biochemistry and cell biology, characterizations of stability and molecular interactions call for a quantitative approach with a level of precision that matches the fine tuning of these interactions in a living cell.

Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists.

This volume provides in a conveniently accessible package a comprehensive collection of accurate and timely information on the management of patients with diarrhea, both in pediatric age and in the adult. As medical knowledge has recently expanded in this area, this volume is full of new practical, clinically useful material for the busy clinician.

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E.

Despite the tremendous diversity of the cells of the hematopoietic system, they are all derived from common precursor cells that are generated in the fetus and persist into adult life. However, new technologies allow increasingly elegant and informative studies in mammalian systems, particularly for cells of the hematopoietic system.

With the cloning of the green fluorescent protein (GFP) from Aequorea victoria in 1992, another valuable tool was added to the arsenal. In Green Fluorescent Proteins: Applications and Protocols examples of how GFP can be utilized in a variety of fields are presented.

This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells.

In this book expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing novel approaches to the production of food, feed, fuel, fiber and pharmaceuticals.

Clinical management of bladder cancer is challenging because of the heterogeneity among bladder tumors with respect to invasion and metastasis, frequent occurrence of new tumors in the bladder among patients treated with bladder preservation treatments and poor prognosis of patients with tumors that invade the bladder muscle and beyond.

The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings.

Determination of the protein sequence is as important today as it was a half century ago, even though the techniques and purposes have changed over time. The first edition of Protein Sequencing Protocols was a "snapshot" of methods in use in protein biochemistry laboratories at the time, and this, the second edition, is likewise.

Parasitic diseases remain a major health problem throughout the world, for both humans and animals. Parasites have complicated life cycles, and a thorough understanding of the unique characteristics of a particular parasite species is vital in attempts to avoid, prevent, or cure infection or to alleviate symptoms.

Internationally recognized investigators review the latest developments in, and novel approaches to, understanding the prion protein and prion diseases at the molecular level.

The study of the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) infections encompasses many different disciplines, including clinical microbiology, diagnostics, animal ecology, and food safety, as well as the cellular microbiology of both bacterial pathogenesis and the mechanisms of toxin action.

Leading biostatisticians and biomedical researchers describe many of the key techniques used to solve commonly occurring data analytic problems in molecular biology, and demonstrate how these methods can be used in the development of new markers for exposure to a risk factor or for disease outcomes.