Bücher veröffentlicht von Humana Press Inc.

Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications.

This volume explores the considerable efforts that have been directed towards the development of G Protein-Coupled Receptors (GPCR) screening assays in order to disclose GPCR acting compounds, elucidate signaling mechanisms or evaluate compound's efficacy.

This book provides current glycoinformatics methods and protocols used to support the determination of carbohydrate structures in biological samples as well as carbohydrate structure databases, the interaction of carbohydrates with proteins, and theoretical and experimental methods to study their three-dimensional structure and dynamics.

Immunocytochemistry and Related Techniques

This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications.

This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology. In the past 20 years, this field has witnessed a remarkable convergence and synergism from information gained using genetic approaches in yeast cells, and biochemical and molecular approaches in mammalian cells.

Integrative Therapies in Lung Health and Sleep provides an overview of integrative therapies to assist clinicians caring for patients with acute or chronic lung diseases and sleep disorders--emphasizing the scientific bases for these therapies;

This book reviews the latest bioabsorbable devices for surgical operations, including surgical adhesives, barriers for the prevention of tissue adhesion, polymers for fractured bone fixation, growth factors for the promotion of wound healing and sutures.

While chest radiography continues to serve as the primary tool for imaging the chest, advances in computed tomography (CT) have led to a variety of applications such as high-resolution CT (HRCT), advanced 3-D airway imaging, and image-guided procedures.The aim of this book is to deliver a clinically-oriented approach to pulmonary imaging.

Ribozymes details protocols and techniques commonly used to study individual ribozymes and their characterizations. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and troubleshooting tips.

Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research.

Erectile dysfunction can affect all age groups. Comprehensive and state-of-the-art, Contemporary Treatment of Erectile Dysfunction: A Clinical Guide synthesizes the literature and covers all aspects of treating erectile dysfunction and other related male sexual dysfunctions.

This book offers basic technical information on controlled genetic manipulation, with examples of creative implementation of the methodology. Covers use of Cre-recombinase, methods for introducing genetic material into the brain, use of optogenetics and more.

This selection of protocols and procedures includes the latest techniques and shows how to make use of cellular, tissue, and whole animal models which can then be applied to the investigation of neurotrophic factors and other agents impacting on these systems.

This exploration of the culture and genetic manipulation of primary fungal pathogens, as well as interactions in model systems, includes detailed, lab-ready techniques as well as the key tips and implementation advice necessary to achieve successful results.

Transcriptomics and proteomics are increasingly deployed in a variety of investigations, including research into brain disorders. Cutting-edge and highly practical, this book sets out easily reproducible methods of both gene- and protein expression profiling.

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states.

Structure-Based Drug Discovery presents an array of methods used to generate crystal structures of biological macromolecules, how to leverage the structural information to design novel ligands anew, and how to iteratively optimize hits and convert them to leads.

The 3rd edition of this Methods in Molecular Biology(TM) book offers detailed protocols and practical advice on modern approaches to the study of leucocytes and their products. Includes materials lists, step-by-step lab protocols, and key tips on troubleshooting.

Genetic testing has become commonplace, and clinicians are frequently able to use knowledge of an individual's specific genetic differences to guide their course of action.

From the world renowned Fred Hutchinson Cancer Research Center, this book is written for all physicians who treat patients with acute or chronic leukemias or myelodysplasia.

International biobank collaborations allow for studies with large number of subjects where generalizability of findings across populations can be investigated, which means establishing quality criteria concerning the nature of the sample, conditions of sample storage, and the adequacy of available information is of vital importance.

Data mining provides a set of new techniques to integrate, synthesize, and analyze tdata, uncovering the hidden patterns that exist within.

A critical review by prominent experts of both the cutting-edge of stem cell biology and the application of stem cells to endocrine diseases, including diabetes, infertility, and liver-associated metabolic disorders.

The advent of non-invasive imaging technology, such as magnetic resonance imaging (MRI), has allowed biologists and clinicians to make great strides in unraveling the secrets of the brain.

Genetic recombination, in the broadest sense, can be defined as any process in which DNA sequences interact and undergo a transfer of information, producing new "recombinant" sequences that contain information from each of the original molecules.

Scientists investigating germ cells have, over the past 15 years, originated discoveries and innovations that give us valuable insights into the mechanisms that regulate not just stem cell function, but human development in its widest sense.

Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.

Written in the popular Methods in Molecular Biology(TM) series format, this book presents a collection of techniques aimed at bridging the translational gap in muscle gene therapy between the prevalent rodent models and vitally important larger animal models.