Bücher veröffentlicht von Humana Press Inc.

This detailed volume explores numerous protocols that can be specifically used for studying plant programmed cell death (PCD), a mechanism involved in a number of physiological and pathological processes that are triggered by developmental requirements as well as changing/adverse environmental conditions.

This book covers the concepts of molecular medicine and personalized medicine.

This third edition provides a wide range of different technologies, ranging from conventional growth basic techniques, application of molecular biology, development of resistance mutations, and diagnosis and monitoring treatment response.  New and updated chapters cover techniques from the microscopic scale to whole animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antibiotic Resistance Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

This volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases.

This volume discusses techniques to synthesize and functionalize nanoparticles, monitor their delivery and uptake, and identify and evaluate their lethal and non-lethal effects on the metabolic activity of the nervous system.

This volume explores the role of proteases in various catalytic classes and cellular processes associated with cancer. The chapters in this book cover topics such as encoding proteolytic enzymes, cleavage sites of proteases, functional catalytic domains of MMPs, zymography,  angiogenesis and lymphangiogenesis, genetic and epigenetic alterations of metallopeptidases, and methods to target metalloproteinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting edge, Proteases and Cancer: Methods and Protocols is a valuable resource for clinical researchers across numerous fields such as molecular and cellular biology, oncology, biochemistry, and chemistry. This book is also useful for PhD students in the various disciples of health sciences.

This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico.  Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field.

This volume provides a broad spectrum of research models, techniques, and protocols. Chapters are divided into sections providing detailed information on the background and context for the chosen topic of interest, a list of the necessary materials and reagents needed for each topic, as well as the step-by-step laboratory protocols and methodologies for the successful and reproducible execution of each topic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Inflammation and Cancer: Methods and Protocols aims to provide information to students and researchers at all levels of experience for further study into this vital field.

This book is organized into 7 parts: Part 1 covers the basics of MRI physics, relaxation, image contrast, and main acquisition sequences; Parts 5 and 6 discuss MRIs and MRSs in animal models of disease and the applications used to study them, and Part 7 looks at anesthesia and advanced contrast agents.

This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies.

This book comprises protocols describing systems biology methodologies and computational tools, offering a variety of ways to analyze different types of high-throughput cancer data. Chapters give an overview over data types available in large-scale data repositories and state-of-the-art methods used in the field of cancer systems biology.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cancer Systems Biology : Methods and Protocols aims to ensure successful results in the further study of this vital field.The chapters "Identifying Genetic Dependencies in Cancer by Analyzing siRNA Screens in Tumor Cell Line Panels", "Perseus: A Bioinformatics Platform for Integrative Analysis of Proteomics Data in Cancer Research" and "Phosphoproteomics-based Profiling of Kinase Activities in Cancer Cells" are available open access under a CC BY 4.0 license via link.springer.com.

This volume discusses the latest advancements and technologies used in cancer drug resistance research. Cancer Drug Resistance: Overviews and Methods contains chapters that cover topics such as: studying the mechanics of resistance to DNA damaging therapeutic drugs; studies to delineate the role of efflux transporters; expression of drug transporters; resistance to targeted therapies in breast cancer; the role of microRNAs in current pancreatic cancer treatment; and cancer exosomes as mediators of drug resistance or clinical and molecular methods in drug development and the use of bioinformatics in the management of cancer drug resistance data. Written in the highly successful Methods in Molecular Biology series format, chapters include overviews of the main issues in cancer drug resistance and the respective mechanisms, as well as introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cancer Drug Resistance: Overviews and Methods, is a valuable resource to researchers, oncobiologists and clinical oncologists or anyone else who is interested in the study of cancer and its drug resistances.

This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute kidney injury, the sympathetic nervous system, and vascular calcification.

This volume presents an overview of contemporary quantitative proteomics methods along with instructions on data interpretation, while providing examples on how to implement proteomics into systems biology.

This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease.

This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology.

This volume guides readers through the field of systems medicine by defining the terminology, and describing how established computational methods form bioinformatics and systems biology can be taken forward to an integrative systems medicine approach.

This volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Chapters in the book cover a wide array of topics such as: quantitation of Flecainide, Mexiletine, Propafenone, and Amiodarone in Serum or Plasma; quantitation of total Buprenorphine and Norbuprenorphine in Meconium; quantitation or Carisoprodol and Meprobamate in Urine; and quantitation of Tricyclic Antidepressants in Serum. Each chapter contains a brief introduction to the topic, clinical utility of the analyte(s), and useful notes to help laboratorians easily reproduce the protocols discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and thorough, Clinical Applications of Mass Spectrometry in Drug Analysis: Methods and Protocols, is a great resource for laboratorians who are already using mass spectrometry or thinking of introducing this technology to their laboratories.

Can microRNAs be useful to regeneratediseased muscle? RegenerativeMedicine for Degenerative Muscle Diseases is ideal for scientistsand clinicians from varying disciplines in genetics, cell biology, virology,cell-based manufacturing, rehabilitation medicine, nutrition, veterinarymedicine and neurosurgery.

This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, P-Type ATPases: Methods and Protocols aims to ensure successful results in the further study of this vital field of research.

This second edition volume brings together the experiences of leading scientists in the discipline of cancer gene profiling. Because cancer genes can be profiled in many different ways, Cancer Gene Profiling: Methods and Protocols, Second Edition explores different techniques and approaches needed to understand the key stages of cancer development, as using only one technique would be insufficient. This book provides readers with an overview of the state-of-the-art methods that will enable them to perform these experiments, and demonstrates the kind of analysis that is possible in our current biomedical research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Cancer Gene Profiling: Methods and Protocols, Second Edition is a great resource for any student or practitioner with an interest in cancer gene profiling, and can be used in any well-equipped research laboratory.

This volume covers a large area, from the description of methodologies for data analysis to the real application. Chapters focus on methodologies for preprocessing of microarray data, a survey of miRNA Data analysis, Cloud-based approaches, application of data mining techniques for data analysis, biclustering to query different datasets, web-based tool to analyze the evolution of miRNA clusters, application of biclustering to mine patterns of co-regulated genes ontologies, microarray and proteomic Data, Gene Regulatory Network Inference, Gene Regulatory Network methods, analysis of Mouse data for metabolomics studies, analysis of microRNA data in Multiple Myeloma, microarray data analysis in Gliobastomas, and microRNA data in Cardiogenesis.Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Microarray Data Analysis: Methods and Applications, Second Edition aims to ensure successful results in the further study of this vital field.

Chapters are split into five parts covering several different aspect of plant cell division such as, cell cultures for cell division studies, cell cycle progression and mitosis, imaging plant cell division, cell division and morphogenesis, and cytokinesis.

This volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis.

This valuable collection aims to provide a collection of frequently used statistical methods in the field of proteomics. Although there is a large overlap between statistical methods for the different ¿omics¿ fields, methods for analyzing data from proteomics experiments need their own specific adaptations. To satisfy that need, Statistical Analysis in Proteomics focuses on the planning of proteomics experiments, the preprocessing and analysis of the data, the integration of proteomics data with other high-throughput data, as well as some special topics. Written for the highly successful Methods in Molecular Biology series, the chapters contain the kind of detail and expert implementation advice that makes for a smooth transition to the laboratory.Practical and authoritative, Statistical Analysis in Proteomics serves as an ideal reference for statisticians involved in the planning and analysis of proteomics experiments, beginners as well as advanced researchers, and also for biologists, biochemists, and medical researchers who want to learn more about the statistical opportunities in the analysis of proteomics data.

Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain.

This volume aims to be a collection of essential protocols in iPS cell field. Induced Pluripotent Stem (iPS) Cells: Methods and Protocols guides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols provides protocols that can be used by both experts and novices interested in stem cells.

and pivotal topics, such as the detection of epigenetics modifications during microspore embryogenesis, the in vitro embryogenesis and plant regenerations from isolated zygotes, the synthetics seed production, the induction and maturation of somatic embryos, and the cryostorage of embryogenic cultures (Part V).

This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols. This book contains chapters that cover various aspects of HIV-1 replication and are helpful for investigating the mechanisms of HIV-1 replication or pathogenesis. The chapters are grouped into six sections: HIV early events; HIV-1 RNA structure and RNA-protein interactions; HIV-1 post-integration events; HIV-1 pathogenesis in animal models; tools to study HIV-1 latency; and Neuro AIDS. Chapters are written by research experts who have diligently developed techniques, improved them, simplified them, or applied them to their own HIV research. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, HIV Protocols, Third Edition is a great resource for HIV research, and contains many significant tips and tricks rarely mentioned in standard protocols.

This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Candida Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.