Bücher veröffentlicht von Humana Press Inc.

The chapters in this book include a look at a range of biomaterials including hydrogels and other matrices (natural, synthetic, self-healing) for various applications including drug and gene delivery, surface modification and functionalization of biomaterials.

This volume describes numerous applications of sponge-matrix histoculture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that produce antibodies, and HIV infection.

This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy.

This volume explores databases containing genome-based data and genome-wide analyses. This book covers databases from all eukaryotic taxa, except plants. The chapters describe database contents and classic use-cases, which assist in accessing eukaryotic genomic data and encouraging comparative genomic research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, step-by-step, readily reproducible computational protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Eukaryotic Genomic Databases: Methods and Protocols is a valuable resource for geneticists and molecular biologists who are interested in the latest eukaryotic genomics data.The chapters 'PomBase: The Scientific Resource for Fission Yeast' and 'The Ensembl Genome Browser: Strategies for Accessing Eukaryotic Genome Data' are available open access under a CC BY 4.0 license via link.springer.com.

This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations.

This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation.

This vital collection provides a unique set of techniques to explore the clinical, pathological, and research aspects of the management of patients with esophageal adenocarcinoma. Beginning with an introduction to the basic features of esophageal adenocarcinoma, the book continues with clinical protocols for the management of the cancer, pathological methods for management of the patients and research, as well as protocols for molecular research, which could aid researchers in furthering our understanding of pathogenesis as well as in identifying new targets for the treatment and prevention of adenocarcinoma. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Esophageal Adenocarcinoma: Methods andProtocols is a valuable guide to stimulate specialists from various disciplines in their continuing research into esophageal adenocarcinoma and translating that research into improving the management of this cancer of rising importance.

This volume introduces the reader to the latest experimental and bioinformatics methods for DNA sequencing, RNA sequencing, cell-free tumour DNA sequencing, single cell sequencing, single-cell proteomics and metabolomics.

This book provides a comprehensive view of the methodologies used for the study of liver toxicity encountered throughout the whole life cycle of a drug, from drug discovery, to clinical trial, post-marketing, and even clinical practice.

This volume discusses the importance of imaging, fluid, and genetic biomarkers in detecting the earliest pre-symptomatic stages of Alzheimer's disease (AD). Parts four and five talk about technological advancements in AD diagnosis and ethical considerations in AD research.

This volume presents protocols for Brachypodium genomics in numerous areas ranging from marker development, trait evolution, functional genomics, metabolomics, transcriptomics, genomics, and tilling.

This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation.

This volume covers an array of techniques available for studying peptide-protein docking and design.

This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods andProtocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation.

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis.

This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols and current methods that cover recent breakthroughs in Drosophila research.

This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells.

Preceded by Forensic DNA typing protocols / edited by Angel Carracedo. 2005.

This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models.

This extensive volume explores areas of intense activity related to the very early commitment of stem cells to particular lineages and the progression of differentiation to mature cell stages.

This detailed volume provides a single, valuable reference source formethods that definitively identify and accurately quantify apoptosis. Itcontinues with multi-parametric and phased apoptosis assays for detecting earlyand late apoptosis or distinguishing apoptosis from necrosis and autophagy.

This detailed volume covers a wide variety of techniques either developed specially for plant senescence studies or optimized for studying senescing plants.

This volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases.