Bücher veröffentlicht von Humana Press Inc.

Small molecule microarrays (SMM) were introduced just a decade ago in 1999 and, within a short space of time, have already established themselves as a vibrant, next generation platform for high-throughput screening.

At the intersection of metabolite analysis, metabolic fingerprinting, and metabolomics, the study of metabolic profiling has evolved steadily over the course of time as have the methods and technologies involved in its study.

Since its first application, microdialysis has become incredibly popular to study brain function and has been applied with success in different fields from psychopharmacology, neurobiology, and physiology in animals and also humans.

The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping.

"Recent studies have discovered new known and characterized cytokines, allowing for advancement in miniaturization of micro-analytical methods as well as the extensive development of bio-informatics and nanotechnology.

In view of the numerous failures of clinical trials aimed at improving stroke therapy, the role and potential benefit of experimentally modeling focal cerebral ischemia in rodents has been debated.

Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cancer metastasis/progression. Therefore, investigations on integrins often demand multi-disciplinary approaches, making researchers long for a handy collection of comprehensive and practical protocols that detail experimental methods for studying integrin and related cell adhesion molecule functionality. Integrin and Cell Adhesion Molecules: Methods and Protocols aims to provide readers not only with basic protocols in studying integrin functions, but also with summaries on those state-of-the-art technologies that have been utilized for understanding integrin functionality at the cellular, molecular, structural, and organismal levels. Divided into six convenient sections, this detailed volume covers  basic protocols for the study of integrin and related cell adhesion molecule functionality in vitro, illustrates structural biology approaches for studying integrins and related cell adhesion molecules, focuses on emerging imaging technologies for investigating cell migration, presents strategies to elucidate signaling through cell adhesion molecules, includes experimental techniques to investigate integrin functions at organismal levels in a physiological context, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics. Written in the successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Both experts and non-experts in the scientific community who wish to study cell adhesion moleculesand diagnostics will find Integrin and Cell Adhesion Molecules: Methods and Protocols authoritative, easily accessible, and vastly informative.

Conditions such as oxidative stress and hypoxia, which have a generalized impact on the oxygen metabolism, have been implicated in the genesis of kidney disease. This means that novel approaches targeting these sensors may offer clinical benefits in kidney disease in which oxidative stress and/or hypoxia is a final, common pathway.

Male Infertility: Problems and Solutions provides a summary of state of the art developments in male infertility for both new and experienced practitioners.

In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery.

Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models;

These include imaging techniques that aim to understand receptor localization and trafficking, techniques to measure receptor-ligand interactions, strategies to identify novel ligands and methods to analyze downstream receptor signaling, as well as strategies for genomic and proteomic characterization of receptor repertoires.

Molecular Diagnostics in Dermatology and Dermatopathology presents the basics of molecular biology and molecular diagnostic methods most commonly used in the clinical laboratory, with an emphasis on the concepts and testing most relevant to dermatological diseases.

The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years.

Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants.

In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications.

Structured in three major sections, this detailed volume covers basic bioinformatics frameworks relating to comparative proteomics, bioinformatics databases and tools for proteomics data analysis, and integrated bioinformatics systems and approaches for studying comparative proteomics in the systems biology context.

Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research.

Despite the many milestones in cystic fibrosis (CF) research, progress towards curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature.