Bücher veröffentlicht von Humana Press Inc.

This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field.

This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology. In the past 20 years, this field has witnessed a remarkable convergence and synergism from information gained using genetic approaches in yeast cells, and biochemical and molecular approaches in mammalian cells.

This volume represents a valuable collection of mobile health (mHealth) emerging technologies. Chapters focus on three main areas of mHealth: technologies for in vitro and environmental testing, mHealth technologies for physiological and anatomical measurements and mHealth technologies for imaging.

This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans.

In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more.

This volume focuses on detecting different cellular stresses, measuring pathological consequences within the cell, and investigating the role of cellular stresses in select diseases. In addition, this book reviews the crosstalk between different stress pathways, stress responses during ageing, and targeting stress for regenerative medicine.

This volume on metabonomics provides detailed information on the procedures involved in nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GS-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS).

This volume is essential for geneticists, molecular biologists, biochemists, and medical doctors interested in the use of mouse models in cancer research.

In this volume expert researchers detail in silico methods widely used to study peptides. Chapters focus on integration and application of technologies to analyze, model, identify, predict, and design a wide variety of bioactive peptides, peptide analogues and peptide drugs, as well as peptide-based biomaterials.

Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software.

Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing.

This volume provides an overview of RNA bioinformatics methodologies, including basic strategies to predict secondary and tertiary structures, and novel algorithms based on massive RNA sequencing.

This volume covers the techniques necessary for a successful fragment-based drug design project, beginning from defining the problem in terms of preparing the protein model, identifying potential binding sites, and the consideration of various candidate fragments for simulation.

Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe.

This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications.

This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step.

This authoritative volume reviews clinical, pathophysiological and therapeutic aspects of oxidative and nitrosative stress in different psychiatric disorders such as schizophrenia, bipolar disorder, autism, and attention deficit hyperactivity disorder (ADHD). Twenty-nine comprehensive chapters are divided into three distinct sections: clinical aspects, pathophysiological aspects, and therapeutic aspects. Together, these chapters present the environmental, genetic and neurodevelopmental factors in the generation of oxidative stress in psychiatric disorders, with particular emphasis on the biochemical changes associated with oxidative stress in dopaminergic and glutamate neurotransmission as well as mitochondrial dysfunction in the brain and peripheral cells. Through an investigation of glutamic acid decarboxylase (GAD) abnormalities in schizophrenia, the book provides a coherent framework to account for the impact of oxidative stress on pathological phenomena ranging from cellular to cognitive and clinical aspects. It describes biomarkers of oxidative damage, the role of oxidative stress in numerous abnormalities of biochemical pathways in the pathophysiology of schizophrenia, the development of new investigative techniques, specially neuroimaging, and studies of apoptotic pathways that seem to prove neurodegenerative and neurodevelopmental theories.Written by leading researchers in their fields, Studies on Psychiatric Disorders explores therapeutic approaches with aspects of various antioxidants, cryostimulation, and hyperbaric oxygen treatment in oxidative stress in neuropsychiatric diseases. The volume also discusses the role of antipsychotics in the treatment of schizophrenia on nitric oxide generation and biomarkers of oxidative stress together with the clinical symptomatology. Overall, it proposes that novel therapeutic strategies such as supplementation with antioxidants¿in particular polyphenols, ¿-3 fatty acids orcombination of both¿could be effective for long-term treatment of some neuropsychiatric disorders.

This book provides current glycoinformatics methods and protocols used to support the determination of carbohydrate structures in biological samples as well as carbohydrate structure databases, the interaction of carbohydrates with proteins, and theoretical and experimental methods to study their three-dimensional structure and dynamics.