Bücher veröffentlicht von Humana Press Inc.

This book details current trends and state-of-the-art in cell and gene based therapies. Examples from various organs and diseases illustrate the potential benefit obtained when both therapeutic approaches are combined with delivery strategies.

This Methods in Molecular Biology(TM) book offers detailed coverage of current techniques in network biology and mathematical models of biological systems. Includes step-by-step workflows, as well as key tips on troubleshooting and avoiding known pitfalls.

This book details approaches to better understand signal transduction and the molecular mechanisms underlying cellular signaling. It features step-by-step, readily reproducible laboratory protocols as well as lists of the necessary materials and reagents.

In Peptide Modifications to Increase Metabolic Stability and Activity, expert researchers in the field provide summarized methods for preparation, purification of modified peptides, and assessment of their biochemical activities.

In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology.

This book reviews common techniques used to measure hormones as well as relatively new methods such as tandem mass spectrometry. It features step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing "no new neuron" doctrine and opened the door to the potential for cell replacement therapy.

Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome.

This book details many of the methods that are now commonly used to study transposons. It features step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine.

In miRNomics: MicroRNA Biology and Computational Analysis, expert researchers in the field present an overview of the current state of the art and aim to put the respective areas of research into a larger perspective.

Embryonic Stem Cell Immunobiology: Methods and Protocols covers a variety of relevant topics, such as hematopoietic stem cells derived from ES cells, the interaction of these cells with natural killer cells or with cytotoxic T cells, and specific protocols for the derivation of hematopoietic cells and neuronal cells, to name a few.

Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants.

Yet another contribution to the highly successful Methods in Molecular Biology series, this guide to the techniques of bioluminescent imaging has stepwise protocols on cutting-edge methods widely used in research and medicine to track biological processes.

This fresh edition features new assay variations and the latest protocols for optimizing drug discovery. With expert tips and troubleshooting advice, and revised throughout for this second edition, it covers cutting-edge techniques such as cytochrome P450.

In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved.

This Methods in Molecular Biology book reviews the cloning and production of proteins for structural studies, experimental methods and computational methods and data analysis. Offers materials lists, reproducible protocols, troubleshooting tips and more.

However, as we learn more about gene function, we discover that the context within which a gene is expressed controls the specific function of that gene.

Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution.

This book details methods for the selection of key cellular, molecular and biochemical techniques that are used in studying the many and varied functions of microglia. It features step-by-step, readily reproducible laboratory protocols.

Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades.

In the second edition of Cell-Cell Interactions: Methods and Protocols, expert researchers in the field detail a collection of protocols to examine interactions between cells. New updated chapters also bring together many currently used assays in examining cell-cell interactions and the biological consequences of those interactions.

From basic performing of sequence alignment through a proficiency at understanding how most industry-standard alignment algorithms achieve their results, Multiple Sequence Alignment Methods describes numerous algorithms and their nuances in chapters written by the experts who developed these algorithms.

Covering standard and recently developed methods in functional analysis of genetic material, this new volume in the Methods in Molecular Biology series includes reproducible protocols, expert problem-solving tips, and improved techniques for histone analysis.

Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology.

In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.

These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques.

This book provides both clinicians and scientists with technical information and extensive background information on the wide ranging approaches available in the diagnostics of melanoma. It features step-by-step, readily reproducible laboratory protocols.