Bücher veröffentlicht von Humana Press Inc.

This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Writtenin the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Thermogenic Fat: Methods and Protocols is a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions.

Thissecond edition details new emerging areas of zebrafish research focusing on geneticsand genomics, techniques for developing and analyzing zebrafish disease models,and methods for neuroscience. Zebrafish:Methods and Protocols, Second Edition guides readers through methods for mutagenesisand genome editing in zebrafish, applicationsof GFP-expressing transgenic fish, techniques for cancer models, imaging of infectionand host-pathogen interactions, metabolism and transport of lipids, and the structure and function of neural circuitsand their role in generating behavior. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls. Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a usefulcomplement to the first book for new and experienced zebrafish researcheralike.

Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Authoritative and practical, Neurotransmitter Transporters: Investigative Methods aims to ensure successful results in thefurther study of this vital field.

This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA.

Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term.

This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using 'omic approaches.

Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.Authoritative and practical, Experimental Neurosurgery in Animal Models aims to ensure successful results in the further study of this vital field.

This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques.

This detailed volume gathers basic and advanced methods and protocols from in vitro assays and in vivo models to address the molecular and functional aspects of tau physiopathology.

This volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches.

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols.

Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology.

In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules.

Updated chapters included in this edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model organisms, and medical genetics and drug discovery.

This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico.

This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells.

"Humana Press is a brand of Springe"--Tp verso.

Mast Cells: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed reviews that cover topics of interest to mast cell neophytes and cognoscenti alike.  With new chapters on methods for the enumeration of tissue mast cells and isolation of mature mast cells and mast cell progenitors from mammalian tissues, Danio rerio (zebrafish) model in the study of mast cell development and function, the functions of mast cells in human health and disease and methods for the isolation, methods for the investigation of the molecular mechanisms of mast cell activation and their effector functions, assays for the detection and analysis of mast cell secretory and cell surface phenotype and, mast cell activation state, and experimental mouse models of disease that have been deemed useful for the assessment of mast cell functions in the regulation of innate and adaptive immune response in cancer, tissue fibrosis, auto-inflammation and allergicdisease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Mast Cells: Methods and Protocols, Second Edition provides a sampler of useful methods and techniques in the further study of mast cell biology.

This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes.

A comprehensive overview of nicotinic receptors that addresses their history from crystal structure to behavior as well as their implications in disease and potential as therapeutic targets.

RNA Interference: Challenges and Therapeutic Opportunities provides readers with recent advances in siRNA design, delivery, targeting and methods to minimize siRNA's unwanted effects.

Covering topics from individual molecules to systemic diseases, from basic concepts to advanced technologies, Pharmacogenomics in Drug Discovery and Development , Second Edition provides a practical, state-of-the-art and integrative view of the application of pharmacogenomics in drug discovery and development.