Bücher veröffentlicht von Humana Press Inc.

Glycosaminoglycans: Chemistry and Biology emphasizes several areas of glycosaminoglycan research especially analysis and application of GAGs using a variety of approaches.

Some are specific for plant research, but most can be adopted for research in other organisms.Authoritative and practical, Polyadenylation in Plants: Methods and Protocols provides scientists with a wide range of methods to study mRNA 3'-end formation in plants.

Hox Genes: Methods and Protocols explores techniques and methodologies which arose from or were successfully applied to the study of Hox genes and Hox proteins, at the intersection of experimental embryology, genetics, biochemistry, physiology, evolutionary biology and other life sciences.

Neurointervention in the Medical Specialties is a first-of-its-kind reference that serves as a bridge between the neurointerventionalist and the physicians who most frequently look to these specialists for answers to some of the most intractable problems they face.

Regulatory Non-Coding RNAs: Methods and Protocols offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression.

This volume highlights the role of proteostasis in human health and associated disease model systems, reflecting its rising importance which has led to the development of new technologies to obtain insight into underling protein mechanistic events.

Plant Proteostasis: Methods and Protocols is broken into four parts focusing on thestudy of ubiquitin-dependent post-translational modifications, protocolsfocused on Ubl post-translational modifications, protein homeostasis, andprotocols for the in silico analysis.

This volume provides current methods and protocols for geneand protein delivery based on both lentivirus-generated and spontaneouslyreleased nanovesicles.

This book is an important new resource for clinicians caring for ventilator dependent children, who often have complex health care needs, are supported by advanced technology and are at high-risk of serious complications.

This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs).

This volume provides a collection of protocols aimed toward the study of ethylene signaling in plants. Ethylene Signaling: Methods and Protocols is divided into three sections: ethylene biosynthesis, the signal transduction pathway, and the diverse ethylene responses of dicots and monocots. The chapters in section one discuss techniques for the measurement of activities related to the biosynthetic enzymes ACC synthase and ACC oxidase, the levels of ethylene synthesized by plants, and the treatment of plants with exogenous ethylene. Section two focuses on the analysis of the new membrane-associated proteins involved in the initial perception and transduction of the ethylene signal, such as ethylene receptors, CTR1, and EIN2. The third section covers assays applicable to dicots and monocots, including methods related to the roles of ethylene in germination, growth, abscission, abiotic stress, and defense. Section three also includes information on Arabidopsis mutantsand the variety of chemical inhibitors that affect ethylene responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and comprehensive, Ethylene Signaling: Methods and Protocols is a valuable resource for both experienced and beginner researchers with prior experience in the study of ethylene signaling and for those who are just entering this exciting research field.

This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting.

This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function.

Practical and cutting-edge, Plant Synthetic Promoters: Methods and Protocols covers all steps required from the identification of cis-regulatory elements, over synthetic promoter design, to the experimental analysis of synthetic promoter function.

Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study.