Bücher veröffentlicht von Humana Press Inc.

Can microRNAs be useful to regeneratediseased muscle? RegenerativeMedicine for Degenerative Muscle Diseases is ideal for scientistsand clinicians from varying disciplines in genetics, cell biology, virology,cell-based manufacturing, rehabilitation medicine, nutrition, veterinarymedicine and neurosurgery.

This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology.

T-Cell Development

Divided into three parts this volume summarizes the most important areas of Cell-Penetrating Peptides (CPP) research . Part one briefly presents the historical background of CPP studies and the classifications of the available CPPs, and then summarizes the approaches for prediction of novel CPPs.

Leading investigators provide up-to-date explanations of laboratory protocols commonly used in diabetes research. Covering in vivo and in vitro model systems, the volume includes an overview of how cellular dysfunction and degeneration leads to diabetes.

The volume is divided into three sections, which cover the transduction of information from mtDNA to functionally active respiratory complexes, mitochondrial reactive oxygen species (ROS) production as well as mtDNA damage and its repair, and the identification and quantification of heteroplasmic mtDNA mutations.

This fresh edition features new assay variations and the latest protocols for optimizing drug discovery. With expert tips and troubleshooting advice, and revised throughout for this second edition, it covers cutting-edge techniques such as cytochrome P450.

Viral Vector Approaches in Neurobiology and Brain Diseases

Bringing together techniques and methods currently being applied to the study of exocytosis, Exocytosis Methods collects chapters from experts in the field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission.

The role of Bioactive Dietary Factors and Plant Extracts in Preventive Dermatology provides current and concise scientific appraisal of the efficacy of foods, nutrients, herbs, and dietary supplements in preventing dermal damage and cancer as well as improving skin health.

In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories.

Gene therapy has acquired a huge amount of new pre-clinical and clinical data. This book fully updates the first edition with thorough coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy.

Initially an application for mRNA, DNA microarray technology is now used for many applications, some from large commercial providers. This book explores those applications and includes details to design and produce one's own probes and arrays.

Due to the importance of potassium channels in cellular function, researchers have been challenged to develop tools and experimental procedures to probe the function and identify chemicals that modulate the behaviour of this super-family of ion channels.

Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace.

Expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping.

In this book, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes.

This book examines various techniques currently used in the rapidly-developing field of neural transplantation for CNS repair. From the laboratory to the clinic it provides thorough and essential protocols to aid current and future neuroscientists.

Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life.

Population studies and epidemiology facilitate the discovery of genetic and environmental determinants of cancer and the development of new approaches to cancer control and prevention, therefore they play a central role in the creation of health policies.

Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway.

Advanced Protocols in Oxidative Stress I covers the field of oxidative stress with state-of-the-art technology to utilize in research, contributed by an international panel of experts renowned for developing new procedures and methods.

Here, experts provide detailed protocols for establishing in vitro cultures of key medicinal plants and their mass multiplication in a controlled environment, as well as numerous other methodologies including large-scale bioreactor metabolite production.

Gene expression can mean the difference between health and disease, the difference between survival and starvation. This third edition brings this vital subject up to date with protocols exploring the most cutting-edge developments in the field.

The radical expansion and revision of techniques and experimental approaches employed in the study of adipose tissue are reflected in this revised book.

In Volume 1, Pathway Methods and Mammalian Models, assays to measure activation of the diverse Wnt signaling pathways are explored, along with a selection of models and strategies used to study mammalian Wnt/FZD fuction, from protein-protein interaction and simple cell line models to organoid cultures and mouse models.

In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols.

This book serves as an easy entry point for those investigating the factors responsible for the pathogenicity of microorganisms. The step-by-step laboratory protocols study host-pathogen interaction, with a focus on fungal, bacterial and parasitic pathogens.

In this volume, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA in order to better treat disease.

In this book, skilled experts provide the most up-to-date, step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo.