Bücher veröffentlicht von Humana Press Inc.

Leading investigators provide up-to-date explanations of laboratory protocols commonly used in diabetes research. Covering in vivo and in vitro model systems, the volume includes an overview of how cellular dysfunction and degeneration leads to diabetes.

This volume presents the techniques to investigate the critical steps involved in the apoptotic process. Coverage includes detection of apoptosis, modifications of apoptotic proteins during apoptosis, and analysis of apoptosis in different organs.

In Protein Networks and Pathway Analysis, expert practitioners present a compilation of methods of functional data analysis, often referred to as "systems biology," and its applications in drug discovery, medicine and basic disease research.

The development of the Western blot has revolutionized biomedical research and medical diagnostics. This book offers numerous protein transfer and detection techniques based on the Western blot and includes step-by-step methods and tips on troubleshooting.

Many questions related to stem cell properties and neural stem cell lineage and differentiation still linger.

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved.

Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles.

In the early years of microarray technology, efforts were directed mainly at profiling expressed genes, while recently the microarray platform has been adapted into diverse applications directed toward the investigation of the physical genome.

Many questions related to stem cell properties and neural stem cell lineage and differentiation still linger.

A comprehensive and authoritative survey of recent findings, ideas, and hypotheses about the causes and treatment of diabetic nephropathy.

A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture.

This volume is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. Attention is given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism.

These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET).

A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease.

A comprehensive review of the use of global gene expression profiling to understand human tumors.

They also delineate the surgical management of the heart patient with discussions of postoperative management and its complications and of specific surgical procedures such as coronary artery bypass grafting, valve surgery, pacemaker and defibibrillators, and surgical management of heart failure.

This volume explores label-free biosensors, advantageous in part because this technology bypasses the need of labels, reporters, and cell engineering, all of which are common to labeled techniques but may introduce artifacts in assay results. Addressing several fundamental and practical aspects as to how to implement label-free methods in the drug discovery process, this book covers a wide range of topics, including binding kinetics determination, fragment screening, antibody epitope mapping, protein-protein interaction profiling and screening, receptor pathway deconvolution, drug pharmacology profiling and screening, target identification, drug toxicity assessment, and physical phenotype profiling and diagnostics based on various cellular processes such as cell adhesion, migration, invasion, infection, and inflammation. As part of the Methods in Pharmacology and Toxicology series, chapters aim to provide key detail and implementation advice to aid with progress in the lab.Practical and thorough, Label-Free Biosensor Methods in Drug Discovery provides a new avenue for rapid access to a focused collection of highly regarded contributions in the field.

They also delineate the surgical management of the heart patient with discussions of postoperative management and its complications and of specific surgical procedures such as coronary artery bypass grafting, valve surgery, pacemaker and defibibrillators, and surgical management of heart failure.

There is also increased coverage of the animal models used in the study of neuroinflammatory mechanisms, of the new imaging methods that allow the noninvasive evaluation of microglial activation in human neurodegernerative disorders, and of the role of neuroinflammation in amyloid-dependent neuronal lysis.

Leading biostatisticians and biomedical researchers describe many of the key techniques used to solve commonly occurring data analytic problems in molecular biology, and demonstrate how these methods can be used in the development of new markers for exposure to a risk factor or for disease outcomes.

A comprehensive review of what is known about the role of cytokines and chemokines in a variety of human infectious diseases, including gram-negative and -positive infections, listeriosis, mycobacterial infections, lyme arthritis, pneumonia, fungal infections, HIV, leishmaniasis, and sepsis.

Cutting-edge and cross-disciplinary, Infectious Causes of Cancer: Targets for Intervention provides clinical oncologists and infectious disease specialists, as well as clinical researchers, with insightful reviews of cancer induction by infectious diseases and the high promise of closely targeted new therapeutics and vaccines.

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease.

Philip Rosenthal, MD, and a panel of leading malaria experts drawn from academia, the military, and international health organizations survey the latest scientific understanding of antimalarial chemotherapy, emphasizing the molecular mechanisms of resistance and the description of important new targets.

Drawing on the highly successful first edition, this newly-revised second edition covers the many advances made in PCR technology since the first book, which has been used in more than 10,000 laboratories worldwide.

The book's many distinguished contributors illuminate the biology and genetics of breast cancer, including what is known about the hereditary breast cancer genes, BRCA1 and 2, the cutting-edge cytogenic approaches, and the biology of breast cancer metastasis.

Leading gene therapy researchers and clinicians illuminate the field-from basic vector technology to current and future clinical applications in neurology.

Cardiac Markers is a comprehensive, up-to-date summary of clinical information on serum cardiac markers for coronary artery disease. Anyone who needs to know how to examine, diagnose, treat, and manage patients with acute chest pain and heart attacks will find this book indispensable.

In David Cope's strikingly intense new collection, Fragments for the Stars, we see the continued development of a highly original art.

This volume provides researchers with up-to-date protocols and takes aclose look at current research and promising applications.