Bücher veröffentlicht von Springer US

This detailed volume provides methods that can be used to study dendritic cell (DC) ontogeny, isolation, migration, and functions. After an introduction to murine and human DC subsets and their unique transcriptional, phenotypic, and functional properties, the book continues with sections covering in vivo studies, in vitro differentiation, enrichment, functional characterization, as well as Omics approaches to study dendritic cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Dendritic Cells: Methods and Protocols is an ideal guide to familiarize readers with the current state of the art techniques to investigate these vital cells.

This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \Cutting-edge and authoritative, Glycosaminoglycans: Methods and Protocols, Second Edition is a valuable experimental manual for practicing researchersincluding graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs.

This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various biomaterials and stem cells, stem cell-derived, or primary vascular cells; biomaterial modification by anticoagulation molecules; vascular bioengineering technologies such as 3D bioprinting; and fabrication of TEVGs with different geometry and multiphase structures. This book also features protocols for grafting and evaluation of vascular grafts in animal models, vascular imaging in animals, and the quantification of blood vessel permeability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tipson troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vascular Tissue Engineering: Methods and Protocols is a valuable resource for biomedical engineers, cell biologists, vascular surgeons, doctors, and nurses.

Reuse Techniques for VLSI Design is a reflection on the current state of the art in design reuse for microelectronic systems. To that end, it is the first book to garner the input of leading experts from both research and application areas. These experts document herein not only their more mature approaches, but also their latest research results. Firstly, it sets out the background and support from international organisations that enforce System-on-a-Chip (SoC) design by reuse- oriented methodologies. This overview is followed by a number of technical presentations covering different requirements of the reuse domain. These are presented from different points of view, i.e., IP provider, IP user, designer, isolated reuse, intra-company or inter-company reuse. More general systems or case studies, e.g., metrics, are followed by comprehensive reuse systems, e.g., reuse management systems partly including business models. Since design reuse must not be restricted to digital components, mixed- signal and analog reuse approaches are also presented. In parallel to the digital domain, this area covers research in reuse database design. Design verification and legal aspects are two important topics that are closely related to the realization of design reuse. These hot topics are covered by presentations that finalize the survey of outstanding research, development and application of design reuse for SoC design. Reuse Techniques for VLSI Design is an invaluable reference for researchers and engineers involved in VLSI/ASIC design.

Continuous Media Databases brings together in one place important contributions and up-to-date research results in this fast moving area. Continuous Media Databases serves as an excellent reference, providing insight into some of the most challenging research issues in the field.

This book provides an analysis of transmission power and network performance in different wireless communication networks. It presents the latest research and techniques for power and interference control and performance modeling in wireless communication networks with different network topologies, air interfaces, and transmission techniques. While studying the power distributions and resource management, the reader will also learn basic methodology and skills for problem formulations, can ascertain the complexity for designing radio resource management strategies in modern wireless communication networks, thus keeping pace with state-of-the-art research progress in radio transmission technologies.

The articles presented here were selected from preliminary versions presented at the International Conference on Genetic Algorithms in June 1991, as well as at a special Workshop on Genetic Algorithms for Machine Learning at the same Conference. Genetic algorithms are general-purpose search algorithms that use principles inspired by natural population genetics to evolve solutions to problems. The basic idea is to maintain a population of knowledge structure that represent candidate solutions to the problem of interest. The population evolves over time through a process of competition (i.e. survival of the fittest) and controlled variation (i.e. recombination and mutation). Genetic Algorithms for Machine Learning contains articles on three topics that have not been the focus of many previous articles on GAs, namely concept learning from examples, reinforcement learning for control, and theoretical analysis of GAs. It is hoped that this sample will serve to broaden the acquaintance of the general machine learning community with the major areas of work on GAs. The articles in this book address a number of central issues in applying GAs to machine learning problems. For example, the choice of appropriate representation and the corresponding set of genetic learning operators is an important set of decisions facing a user of a genetic algorithm. The study of genetic algorithms is proceeding at a robust pace. If experimental progress and theoretical understanding continue to evolve as expected, genetic algorithms will continue to provide a distinctive approach to machine learning. Genetic Algorithms for Machine Learning is an edited volume of original research made up of invited contributions by leading researchers.

Robotic Renal Surgery: Benign and Cancer Surgery for the Kidneys and Ureters provides a comprehensive review of the role of and technical considerations regarding robotic surgery for conditions of the kidney and associated conditions of the upper urinary tract.  In addition to serving as a reference regarding indications, preoperative and postoperative management, complications, and evidence-based outcomes, this text also serves as a practical guide for surgeons in how to perform the complete array of  robotic kidney and upper tract surgery.  Included are detailed descriptions of positioning, instrumentation, and surgical steps for the surgeon newly adopting robotic surgery or for those refining their techniques.  All chapters are written by recognized and published experts in the various techniques, creating an authoritative text on the subject.  Robotic Renal Surgery: Benign and Cancer Surgery for the Kidneys and Ureters will be of great value to urologists, robotic surgeons, fellows in urologic oncology or endourology as well as urology residents in training and surgical nurses and other surgery team members involved in these procedures.  ¿

¿Information Systems (IS) as a discipline draws on diverse areas including, technology, organisational theory, management and social science. The field is recognized as very broad and encompassing many themes and areas. However, the development of artefacts, or information systems development (ISD), in the broadest sense, is a central concern of the discipline. Significantly, ISD impacts on the organisational and societal contexts through the use of the artefacts constructed by the development. Today, that impact also needs to be evaluated in terms of its effects on the environment. Sustainable, or "green," IT is a catch-all term used to describe the development, manufacture, management, use and disposal of ICT in a way that minimizes damage to the environment. As a result, the term has many different meanings, depending on the role assumed in the life span of the ICT artefact. The theme of the proposed work is to critically examine the whole range of issues around ISD from the perspective of sustainability. Sustainable IT is an emerging theme in academic research and industry practice in response to an individual concern for the environment and the embryonic regulatory environments being enacted globally to address the environmental impact of ICT. In this work we intend to bring together in one volume the diverse research around the development of sustainable IS.

Cardiovascular drug therapy has markedly progressed in the recent decades. Not only have new drugs been introduced to clinical practice, but new classes of drugs have been developed. While in 1960 the practicing cardiolo­ gist had a selection of about only ten drugs, in 1987 about 150 drugs are routinely used in cardiovascular diseases. Elderly patients, however, usually do not enjoy the full benefit of this progress. This might be due to lack of knowledge, a conservative approach, or the worldwide tendency not to try new drugs in the elderly. It is now clear that the majority of patients that will be treated in car­ diovascular clinics will be, in the near future, elderly patients. Even now, elderly patients form about one-third of the patients with cardiovascular diseases. These patients are approached, however, according to criteria devel­ oped for younger populations. This is despite the fact that elderly patients differ from younger ones in most aspects, including pathology, epidemiol­ ogy, pathophysiology, diagnostic approach, management, pharmacology, pharmacokinetics, rehabilitation, and supportive treatment. It is the purpose of this book to present to the clinician all drugs with which there is clinical experience in the elderly or which might be potentially useful for the elderly with cardiovascular diseases. The data are presented without the authors taking a position. This should allow the clinicians to make their own selection and individualize treatment, vii viii Preface based on a wide data base. Comparative data are presented only when specific comparative studies were performed.