Bücher veröffentlicht von Springer US

The book provides the readers of various discipline an easy understanding of the latest biophysical techniques pertaining to microbiology. Biofilm associated chronic infection is a major health problem and a serious concern to doctors, scientists and other health workers as it develops antibiotic and multi-drug resistance. This book describes various protocols utilized in the detection of the biofilm. The book has been divided into six sub sections which provides pertinent information about the various biophysical techniques and instruments that are used for detecting and analyzing the biofilm formation upon biotic and abiotic surfaces. The readers will be able to identify the techniques that can best cater information to solve the problem at hand. This book attempts to compile the latest information on the recent advances in the various functional aspects of microbial biofilms, their pathogenesis, present day treatments as well as detection strategies.This book is meant forresearchers in the field of microbiology and interested in understanding microbial pathogenesis, quorum sensing and biofilm formation.

This is a review of recent advances on the use of DNA microarray for diagnosing foodborne pathogens. Rapid detection and characterization of foodborne pathogens is critical for food safety. Many relevant technologies have been intensively developed to date. DNA microarray technology offers a new way to food safety involving pathogen detection and characterization. DNA microarray can be used for detection and characterization of pathogens by analyzing hybridization patterns between capture probes and nucleic acids isolated from food samples or bacteria. It allows more rapid, accurate, and cost-effective detection of pathogens compared with traditional approaches of cultivation or immuno-assays. The application of DNA microarrays to different foodborne bacteria, such as Campylobacter, Salmonella, Listeria monocytogenes, or Shiga toxin producing Escherichia coli, will improve their rapid identification and characterization of their genetic traits (e.g., antimicrobial resistance, virulence). As bacterial foodborne diseases are posing more serious threats to public healthcare, development of rapid and accurate methods for pathogen detection and characterization is critical to their proper control at the earliest time.

This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. Authoritativeand practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines.

This thorough book collects methods and strategies to analyze proteomics data. It is intended to describe how data obtained by gel-based or gel-free proteomics approaches can be inspected, organized, and interpreted to extrapolate biological information. Organized into four sections, the volume explores strategies to analyze proteomics data obtained by gel-based approaches, different data analysis approaches for gel-free proteomics experiments, bioinformatic tools for the interpretation of proteomics data to obtain biological significant information, as well as methods to integrate proteomics data with other omics datasets including genomics, transcriptomics, metabolomics, and other types of data. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that will ensure high quality results in the lab. Authoritative and practical, Proteomics Data Analysis serves as an idealguide to introduce researchers, both experienced and novice, to new tools and approaches for data analysis to encourage the further study of proteomics.Chapter 16 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Verilog HDL is the standard hardware description language for the design of digital systems and VLSI devices. This volume shows designers how to describe pieces of hardware functionally in Verilog using a top-down design approach, which is illustrated with a number of large design examples. The work is organized to present material in a progressive manner, beginning with an introduction to Verilog HDL and ending with a complete example of the modelling and testing of a large subsystem.

The idea for this book came about one Friday afternoon towards the end of a summer term. I was giving the third project tutorial of the afternoon. The first had been to a BA (Business Studies) student, the second to a part-time MBA student, and the third to a student registered on the Diploma in Personnel Management programme, and a great variety of issues had been dealt with during the course of the time involved. Nevertheless, I noticed that some of the material was common to all three students. I found myself thinking that I was repeating myself, and wanting to get through the basics as quickly as possible so that we could move on to the specifics of each particular project, which we both, each student and I, found more interesting to deal with. Unfortunately, the basics were precisely those topics which I considered essential to the success of any project. What's more, they dealt with the sort of material which wouldn't, on the whole, have occurred spontaneously to many students, and so it was a necessary part of my job to go through them. One or two could be dealt with by issuing a handout, and the student could be referred to the library for some of the rest, but there wasn't a systematic written compilation of all the points that I needed to make.

Whilst this book is primarily compiled for nurses who care for patients with eye disease, it will be useful to other health professionals who are also interested in the holistic ca re of such patients, e.g. opticians, orthop- tists, social workers and junior medical staff. The care is presented as a systematic approach to the more common actual and potential problems. Typically, a problem is identified with a desired outcome and the nursing interventions possible to achieve this. Evaluation has not been included as this is dependent on an identified time limit for outcomes to be achieved which is individual to each patient. Part One covers the general aspects of ophthalmie care in the various situations where patients are nursed, e.g. casualty, theatre, wards. Part Two is based on a medical model, where each section of the eye is studied separately; the applied physiology of that part of the eye; the more common conditions affecting it; the possible medical management of the condition and the nursing care of the patient taking into account the medical prescription. The list of medical conditions is not exhaus- tive, and only a general guideline of medical management is given as far as it influences nursing care. A list of widely available books for further reference to ocular pathology and medical management is given in the Appendix.

This book provides an introduction to the analysis of multivariate data. It should be suitable for statisticians and other research workers who are familiar with basic probability theory and elementary inference, and also have a basic grounding in matrix algebra. The book should also be suitable as a text for undergraduate and postgraduate statistics courses on multivariate analysis. The book covers a wider range oftopics than some other books in this area. It deals with preliminary data analysis, principal component and factor analysis and traditional normal-theory material. It also covers cluster analysis and scaling techniques. In writing the book, we have tried to provide a reasonable blend of theory and practice, in contrast to much of the existing literature which concentrates on one or other aspect. Enough theory is given to introduce the concepts and to make the topics mathematically interesting. But we also discuss the use (and misuse) of the techniques in practice and present appropriate real-life examples. Although the book is primarily an introductory text, we have nevertheless added appropriate references for further reading, so that the reader may extend his studies if he wishes.

The ftavonoid pigments, one of the most numerous and widespread groups of natural constituents, are ofimportance and interest to a wide variety ofphysical and biological scientists and work on their chemistry, occurrence, natural distribution and biological function continues unabated. In 1975, a mono graph covering their chemistry and biochemistry was published by Chapman and Hall under our editors hip entitled The Flavonoids. The considerable success of this publication indicated that it filled an important place in the scientific literature with its comprehensive coverage of these fascinating and versatile plant substances. The present volume is intended to update that earlier work and provide a detailed review of progress in the ftavonoid field during the years 1975 to 1980. Although cross references are made to The Flavonoids, this supplement is entirely self-contained and where necessary, tabular da ta from the earlier volume are incJuded and expanded here. The choice oftopics inRecent Advances has been dictated by the developments that have occurred in ftavonoid research since 1975, so that not all subjects covered in The Flavonoids are reviewed again here. A major advance in ftavonoid separation has been the app1ication ofhigh performance liquid chromatography (HPLC) and this is reviewed inter alia in the opening chapter on separation techniques. An equally important development in the spectral analysis of ftavonoids has been the measurement of carbon-13 NMR spectra and this subject is authoritatively discussed in Chapter 2 and is also illustrated with the spectra of 125 representative ftavonoids.

This textbook serves as an introduction to the subject of embedded systems design, with emphasis on integration of custom hardware components with software. The key problem addressed in the book is the following: how can an embedded systems designer strike a balance between flexibility and efficiency? The book describes how combining hardware design with software design leads to a solution to this important computer engineering problem.  The book covers four topics in hardware/software codesign: fundamentals, the design space of custom architectures, the hardware/software interface and application examples. The book comes with an associated design environment that helps the reader to perform experiments in hardware/software codesign. Each chapter also includes exercises and further reading suggestions. Improvements in this second edition include labs and examples using modern FPGA environments from Xilinx and Altera, which will make the material in this book applicable to a greater number of courses where these tools are already in use.  More examples and exercises have been added throughout the book. ¿If I were teaching a course on this subject, I would use this as a resource and text. If I were a student who wanted to learn codesign, I would look for a course that at least used a similar approach. If I were an engineer or engineering manager who wanted to learn more about codesign from a very practical perspective, I would read this book first before any other. When I first started learning about codesign as a practitioner, a book like this would have been the perfect introduction.¿ --Grant Martin, Tensilica--