Bücher veröffentlicht von Springer Verlag, Singapore

This book provides a brief overview of single-cell analysis using recent advanced technologies. The different sections cover different aspect of single cell analysis and applications with their advantages, limitations, and future challenges. The book has covered how different physical energies such as optical, electrical, and mechanical energy have been applied for single cell therapy and analysis. The recent advanced micro/nanofluidic devices have been employed for single-cell counting, manipulation, cultivation, separation, isolation, lysis, printing and patterning and host-viral interaction at single-cell level. Various chemical approaches for single-cell analysis have been discussed, such as liposome mediated materials transfer at single-cell and their analysis, discovery of antibody via single-cell, high-throughput screening of antigen-specific antibody-secreting cells, and biomolecular secretion analysis of individual cells. Moreover, different single-cell omics such as genomics, proteomics and transcriptomics have been discussed using microfluidic technologies as well as conventional approaches.The role of single cell analysis in system biology and biocatalysis have been discussed in detail. The book describes single-cell phenotyping of heterogeneous tissue, stimulation, and instant reaction quenching technology for biochemical kinetic analysis, large scale single-cell assay for the identification of biocatalysts and analytical techniques for single-cell studies in microbiology. The role of single-cell analysis in cancer, such as single-cell adhesion and cancer progression, single-cell technologies for cancer therapy, analytical technology for single cancer cell analysis, and biophysical markers for cancer cell analysis have been discussed. The flow cytometry based high throughput single-cell analysis have been well emphasized. Finally this book has covered single-cell electrophysiology, single-cell sensing and size measurement using mechanical and microwave resonators, molecular force spectroscopy for cell adhesion measurement, micro-tweezers and force microscopy techniques for single-cell mechanobiological analysis, mass spectrometry and acoustic tweezers for single-cell manipulation and analysis. This book is intended for academic and industrial researchers, undergraduate and graduate students in the fields of biomedical engineering, bio-micro/nanoengineering, and bio-micro/nano fabrication for single-cell analysis. It can be used for courses on bio-MEMS/bio-NEMS, biomicrofluidics, bio-micro/nanofabrications, micro/nanofluidics, biophysics, single cell analysis, bionanotechnology, drug delivery systems and biomedical microdevices. Collective contributions from respected experts, have brought diverse aspects of single-cell technologies in a single hand book. This will benefit researchers and practitioners in the biotechnology industry for different diseases analysis, therapeutics, diagnostics, drug discovery, drug screening etc.In addition to hard copies, the book will be available online and will often be updated by the authors.

The book provides a detailed description in color format of the optic nerve head changes that occur in glaucoma. It explains the normal and variant anatomy of an optic nerve head and why certain discs are more or less likely to develop glaucomatous changes.This book attempts to explain the anatomical basis of common and not so common signs seen in optic nerve head and retinal nerve fibre layer as captured by a digital fundus imaging system and optical coherence tomography. It also includes optic nerve head conditions mimicking glaucoma. The book is relevant for practicing ophthalmologists, vision science researchers, clinical optometrists, postgraduate residents in ophthalmology, sub-specialty fellows as well as general ophthalmologists, neurologists and neurosurgeons.

This book covers major knowledge and recent advance regarding portal vein thrombosis. Portal vein thrombosis, a potentially life-threatening vascular disorder of the liver, refers to the development of thrombosis within main portal vein trunk and intrahepatic portal vein branches with or without splenic and mesenteric veins. In patients with acute portal vein thrombosis, if a thrombus extends into the mesenteric veins, there is a risk of intestinal ischemia and necrosis; in patients with chronic portal vein thrombosis, portal hypertension and its complications develop. The book includes chapters on the topics of epidemiology, risk factors, diagnosis, and prognostic impact, pharmacological, interventional with surgical treatment of this disease. Concepts of early diagnosis of portal vein thrombosis and extensive assessment of its severity are fully introduced. The book is beneficial for physicians to establish standard treatment strategy, for researchers to launch creative clinical and experimental studies.

This book amalgamates the basic concepts in understanding the science of maxillofacial skeleton with the clinical skills required towards managing complex facial fractures. The book is presented in two sections. The first section introduces the readers with the introduction to maxillofacial trauma, biomechanics of maxillofacial skeleton, the principle of internal fixation, medicolegal aspects of maxillofacial trauma, and preoperative workup which provides a brief outline towards an understanding of the basic concepts about the anatomy and physiology of facial skeleton. The second section is oriented clinically with case-based discussions that start from the emergency management of facial trauma including the recent protocols of basic life support and advanced trauma life support, emergency airway management followed by definitive management guidelines in stabilizing and fixing the fractured facial bones. The clinical cases have been discussed in a way to provide practical knowledgeand skills to the postgraduate students and clinicians who will enhance their knowledge and facilitate the decision-making process. This book would be a valuable read for clinicians in oral & maxillofacial surgery, ENT surgery, plastic surgery and allied trauma specialists dealing with maxillofacial trauma.

This book introduces the surgical suture techniques in orthopaedics. These techniques have been recognized as a crucial part for wound care and surgery-related prognosis. Training of fellows on suture techniques is of great importance. This book provides a standard tutorial on how to be proficient in surgical suture performance. The history and basic concepts are introduced. Important issues when considering suture methods, including site infections, suturing materials, room setups, cosmetics and drainage are also discussed fully. Different types of suture techniques applying to orthopaedic surgeries are presented with illustrations.The author strives to implement the principle that orthopaedic theory should be connected with clinical practice, highlight the application of theoretical knowledge, strengthen the pertinence and practicality of suture techniques, and reflect domestic and international development trends to the greatest extend.

This book serves as a practical guide for the otorhinolaryngologists to better understand the diseases of the sinonasal region, diagnosis, and management. The detailed knowledge of the complex anatomy of the sinonasal region is the key to surgical success. The text aims to help budding and practicing rhinologists to get an essence abreast of the current scientific advancement by engaging rhinologists with excellent awareness and knowledge as contributors. The book expands its span afar the usual by including topics on complications of endoscopic surgeries, empty sinus syndrome, packing material, open transcranial approach, biofilm, instruments, cavity management, and improved quality of life, etc. The purpose of microbiology, interventional radiology, pathology and nuclear medicine in the diagnosis and management of sinonasal diseases is contributed by authors from allied specialties. This book will be a useful resource for medical students, postgraduates in ENT, practicing rhinologists and general physicians in treating sinonasal diseases.

Clinical Rheumatology is a book written by a clinician for clinicians. It covers all the essential clinical aspects of Rheumatology in an engaging, clear, and concise manner, thereby fulfilling an unmet need. The focus of this book is to cover clinically pertinent and practically relevant issues while pruning unnecessary detail. Patient photographs, tables, and boxes enhance readability. The bedside clinical and investigative approach is discussed in a lucid fashion illustrated by clinical photographs, flowcharts, and algorithms. The evidence-based treatment is spelt out in an easy to comprehend fashion. Key messages have been listed at the beginning of each chapter. The book is intended for undergraduate and postgraduate medical students, residents, fellows, and clinicians who want to gain practical knowledge and clinical insight into rheumatic diseases. The book is likely to appeal to internists, rheumatologists, physiatrists, physiotherapists, occupationaltherapists, as well as orthopaedic surgeons. They will find their day to day questions answered in a knowledge format that can be applied straight away. Senior clinicians will find it a ready reckoner and a handy manual to refresh and update their knowledge. Basic scientists will find it useful to gain clinical insight into the rheumatic diseases they research without being intimidated by the size of the text. Teachers will find it full of helpful teaching messages. Clinical Rheumatology is a must-have book for all those who deal with rheumatic musculoskeletal diseases.

This book describes the fundamentals of microfluidics and fabrication methods of microfluidic devices that can be adopted for animal-assisted reproduction. It presents microfluidic methods for sorting highly fertile spermatozoa. This book also describes the application of microfluidics in vitro fertilization and embryo culture. It discusses the use of microfluidics in sperm sexing and the cryopreservation of animal gametes and embryos. Lastly, the book examines the potential opportunities of microfluidics in infertility diagnosis, sperm selection and guidance, oocyte selection, insemination, and embryo monitoring.