Analysis, selection and determination of the efficacy of disinfectants

In the present work, the efficacy of different disinfectants (70 and 96° ethyl alcohol, 10% hydrogen peroxide and acetone) was determined by varying the contact time with the biological tissue (10 and 20 minutes) and thus being able to select the procedure or alternative that gives the best results in terms of the elimination of unwanted tissue remains as well as the elimination of microorganisms. As a result of the present work, it was obtained that 96° ethyl alcohol for 20 minutes is the treatment that gives the best results, achieving a reduction of 95.2 % of the total microorganisms present in the sample when the procedure was challenged against reference microorganisms. The efficiency of the proposed procedure was then checked by subjecting the osteochondral tissue to the treatment, achieving acceptable microbial count values after the treatment, since after seeding and subsequent incubation, no microbial growth was observed. Therefore, the use of 96° ethyl alcohol for 20 minutes is proposed as one of the possible alternatives for the cleaning and disinfection of bovine osteochondral tissue.