Epigenetic Reprogramming During Mouse Embryogenesis

Preface...
Table of Contents...
Contributing Authors...
1.Ex Vivo Culture for Preimplantation Mouse Embryo to Analyse Pluripotency
Katia Boutourlinsky, Nicolas Allègre, and Claire Chazaud
2.Microfabricated Device for High Resolution Imaging of Preimplantation Embryos
Sandrine Vandormael-Pournin, Emmanuel Frachon, Samy Gobaa, and Michel Cohen-Tannoudji
3. In Vitro Culture of Mouse Blastocyst to the Egg Cylinder Stage via Mural Trophectoderm Excision
Hatice O. Ozguldez and Ivan Bedzhov
4. Spatially Organized Differentiation of Mouse Pluripotent Stem Cells on Micropatterned Surfaces
Sophie M. Morgani and Anna-Katerina Hadjantonakis

5. Mouse Primordial Germ Cells: In Vitro Culture and Conversion to Pluripotent Stem Cell Lines
Malgorzata Borkowska and Harry G. Leitch
6. Generation of Primordial Germ Cell-Like Cells in Small and Large Scales
Wolfram H. Gruhn and Ufuk G├╝nesdogan
7. Genome-Scale CRISPR Screening for Regulators of Cell Fate Transitions
Valentina Carlini, Kristjan H. Gretarsson, and Jamie A. Hackett
8. Somatic Reprogramming by Nuclear Transfer
Vincent Brochard and Nathalie Beaujean
9. Targeted Transgenic Mice using CRISPR/Cas9 Technology
Fatima El Marjou, Colin Jouhanneau, and Denis Krndija
10. Whole-Mount Immunofluorescence Staining of Early Mouse Embryos
Frederick C. K. Wong

11. Investigating the Inner Cell Mass of the Mouse Blastocyst by Combined Immunofluorescence Staining and RNA Fluorescent In Situ Hybridization
Maud Borensztein

12. Mapping of Chromosome Territories by 3D-Chromosome Painting during Early Mouse Development
Katia Ancelin, Yusuke Miyanari, Olivier Leroy, Maria-Elena Torres-Padilla, and Edith Heard
13. Deciphering the Early Mouse Embryo Transcriptome by Low-Input RNA-seq
Raquel Pérez-Palacios, Patricia Fauque, Aurélie Teissandier, and Déborah Bourc''his
14. Studying DNA Methylation Genome-Wide by Bisulfite Sequencing from Low Amounts of DNA in Mammals
Ambre Bender, Hala Al Adhami, Thomas Dahlet, and Michael Weber
15. Profiling DNA Methylation Genome-Wide in Single Cells
Antonio Galvao and Gavin Kelsey

16. Tracking Histone Modifications in Embryos and Low Input Samples using Ultrasensitive STAR ChIP-seq
Bingjie Zhang, Xu Peng, Feng Xu, and Wei Xie
17. Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUN
Srinivasa Abishek Prakash and Joan Barau

18. DamID to Map Genome-Protein Interactions in Pre-Implantation Mouse Embryos
Mrinmoy Pal, Kim de Luca, Jop Kind, and Maria-Elena Torres-Padilla
19. Understanding Chromosome Structure during Early Mouse Development by Single-Cell Hi-C Analysis
Noémie Ranisavljevic, Maud Borensztein, and Katia Ancelin

20. Bioinformatic Analysis of Single Cell Hi-C Data from Early Mouse Embryo
Samuel Collombet, Yuvia A. Pérez-Rico, Katia Ancelin, Nicolas Servant, and Edith Heard

Subject Index List...