Bücher veröffentlicht von Humana Press Inc.

Elizabeth Rakcozy and a team of leading clinical and experimental scientists describe in step-by-step detail the key techniques essential to effective molecular biological research in ophthalmology and optometry.

There are several chapters devoted to the microorganisms associated with fermented foods: these are of increasing importance in food microbiology, and include one bacteriophage that kills the lactic acid bacteria involved in the manufacture of different foods-cottage cheese, yogurt, sauerkraut, and many others.

Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems.

Genomics in Endocrinology focuses on exciting new advances in endocrinology resulting from DNA microarray studies and includes a comprehensive introduction to the use of DNA microarrays in endocrinology. Topics discussed include the methodology of DNA microarrays and general methods for the analysis of microarray data.

This book investigates intraperitoneal chemotherapy in a variety of complex and interesting ways. Authors also examine regional approaches to therapy, systemic therapy, and the use of carboplatin and paclitaxel as the standard treatment for women with stages III and IV ovarian cancer.

Molecular and Cellular Basis of Inflammation was first conceived during a mini symposium sponsored by the American Society for Investigative Pathology held at FASEB in 1995 entitled "The Role of Reactive Lipids, Oxygen and Nitro gen Metabolites in Inflammation," at which several of the contributing authors delivered lectures.

This volume on Asthma: Mechanisms and Protocols in the Methods in Molecular Medicineseries provides an overview of the molecular mechanisms involved in asthma by p- viding extensive protocols that are being used in asthma research.

Putrescine and spermidine are ubiquitous in living organisms. Spermine, third of the three most commonly occurring natural polyamines, is probably present in all eukaryotes but is rare (or nonexistent) in prokaryotes. Polyamine residues are constituents of many compounds found in plants and insects. Putrescine, spermidine, or spermine-containing alkaloids are found in many plants, nonproteinaceous spider and wasp toxins contain polyamine residues, and glutathionyl¿spermidine conjugates have been found in some pathogenic microorganisms. In most cells polyamines are the products of a highly regulated bios- thetic pathway. It is not clear whether the elaborate regulation of polyamine synthesis is a consequence of their essential role(s) in cellular differentiation and development, or part of a defense mechanism to prevent overaccumulation of compounds that are toxic in excess. In addition to their biosynthetic capa­ bility, many cells also possess transport systems for polyamines that respond to intracellular polyamine levels, and other stimuli, and are regulated by mecha­ nisms that are at present incompletely defined. Two routes of polyamine catabolism have been identified in mammalian cells, a biodegradative route and a recycling pathway. The relative impor­ tance of these pathways and their overall regulation is only partially resolved. What is clear is the widespread occurrence of a variety of polyamine-oxid- ing enzymes in animals, plants, bacteria, and fungi. Polyamine catabolism, by whichever route, results in the formation of aminoaldehydes as intermediates.

The frequency of reports concemmg the interface of biological reco- tion elements to signal transduction technologies has risen dramatically over the last decade. 7: Affinity Biosensors: Techniques and Protocols, describes a variety of classical and emerging transduction technologies that have been interfaced to bioaffinity elements (e.

The book's many distinguished contributors illuminate the biology and genetics of breast cancer, including what is known about the hereditary breast cancer genes, BRCA1 and 2, the cutting-edge cytogenic approaches, and the biology of breast cancer metastasis.

General initiation factors are involved in positioning RNA polymerase II on the core promoter, but the complex - teraction of these proteins and transcriptional activators binding to DNA e- ments outside the core promoter regulate the rate of transcriptional initiation.

Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H.

The synthesis of proteins from 20 or so constituent amino acids according to a strictly defined code with an accuracy of better than 1 in 10,000 at most loca tions is arguably the most complex task performed by cells.

It is now more than ten years since Dr. Alec Jeffreys (now Professor Sir Alec Jeffreys, FRS) reported in Nature that the investigation of certain minisatellite regions in the human genome could produce what he termed DNA fingerprints and provide useful information in the fields of paternity testing and forensic analysis.

Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

The unexpected and unp- dictable occurrence of the disease in previously healthy children and young adults, its rapid progression, and the frequent occurrence of purpura fulminans with the resulting gangrene of limbs and digits and the requirement for mutilating s- gery, have all heightened both public and medical interest in the disease.

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis.

In Human Cloning a panel of distinguished philosophers, medical ethicists, religious thinkers, and social critics tackle the thorny problems raised by the now real possibility of human cloning.

Leading researchers and experienced clinicians join forces not only to illuminate today's understanding of the endocrinology of menopause, but also to provide a sound basis for its successful management.

A panel of leading experts integrate the latest findings from basic and clinical science to create a comprehensive treatment of the processes by which the brain acts as an endocrine organ, not only to control hormonal functions, but also to maintain homeostasis and regulate behavior.

Renowned philosophers and medical ethicists debate and discuss the profoundly important concepts of disease and health. Christopher Boorse begins with an extensive reexamination of his seminal definition of disease as a value-free scientific concept.

The distinguished contributors integrate a wide range of topics, including the basic biochemistry of EFAs and lipid metabolism, the role of EFAs in the neuronal membrane, the effects of EFAs and lipids in various diseases, and the effects of normal levels and EFA deficiencies on cognition and behavior.

Recombinant Proteins from Plants is one of the most exciting and fastest developing areas in biology. The latest molecular techniques are being applied to the exploitation of plants as novel expression systems for the p- duction and overproduction of heterologous and native proteins. Transgenic plant technology is currently used in three broad areas: the expression of - combinant proteins to improve crop quality by increasing disease/pest res- tance or increasing tolerance to stress, optimizing plant productivity and yield by the genetic manipulation of metabolic pathways, and the large-scale co- effective production of recombinant proteins for use as specialist industrial or therapeutic biomolecules. The intention of Recombinant Proteins from Plants is to provide c- prehensive and detailed protocols covering all the latest molecular approaches. Because the production oftransgenic plants has become routine in many la- ratories, coverage is also given to some of the more "classical" approaches to the separation, analysis, and characterization of recombinant proteins. The book also includes areas of research that we believe will become increasingly important in the near future: efficient transformation of monocots with Agrobacterium optimizing the stability of recombinant proteins, and a section highlighting the immunotherapeutic potential of plant-expressed proteins.

Continuing the research of the best-selling first edition, this second edition collects three more years of research in the ever-expanding study of the cell membrane.

Leading clinical and basic science researchers present the latest molecular and cellular findings on key gut peptides, illuminating their physiology and pathophysiology, as well as highlighting the regulatory mechanisms underlying their action in the intestinal tract.

Several clinically oriented chapters are significant for neurologists treating Parkinsonism, for psychiatrists treating drug abuse and neurodegenerative disorders, and for primary care physicians treating patients with appetite suppressants.

In this book, leading-edge investigators offer effective strategies to improve current antidepressive therapies and suggest molecular, biological, and genetic approaches that will lead to the development of novel antidepressants.

The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods.