Bücher veröffentlicht von Humana Press Inc.

Bone Pathology is the second edition of the book, A Compendium of Skeletal Pathology that published 10 years ago. Similar to the prior edition, this book complements standard pathology texts and blends new but relatively established information on the molecular biology of the bone.

Gives a treatment of the highly successful use of the 192 IgG-saporin and ME20.4-saporin immunotoxins to lesion the cholinergic basal forebrain in order to model the behavior, anatomy, physiology, and pharmacology of Alzheimer's disease in animals. This book explains the theory and techniques of using targeted toxins experimentally.

A panel of world-renowned experts presents a complete course on evaluating and treating patients with back pain, including interventional spinal procedures, spinal imaging, and the clinical evaluation of the spine patient.

Prominent physicians review past, current, and future applications of the many powerful imaging techniques now used in the diagnosis, staging, treatment, and outcomes assessment of cancers of the prostate, central nervous system (CNS), and breast.

This volume provides insight into recent developments on experimental and clinical strategies for cancer gene therapy. Gene Therapy of Solid Cancers: Methods and Protocols guides readers through protocols on gene therapeutic strategies in combination with helpful technical notes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Concise and easy-to-use, Gene Therapy of Solid Cancers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Pyrosequencing

Providing a spectrum of models that is reflective of the various species that can be utilized in experimentation on disorders across a broad range of developmental disabilities, this volume collects expert contributions involved in investigation of the causes, outcomes, treatment, and prevention.

Asthma is a chronic relapsing airways disease that represents a major public health problemworldwide.

This volume details protocols on detection and analysis of fusion events in various cell types in vitro and in vivo.

However, working with plant tissue imposes particular challenges to the biologist which are attributed to the rigid cell wall making protein extraction more difficult, the skewed protein abundance with Rubisco as a highly abundant protein and a large central vacuole leading to low protein yield and increased degradative enzyme activity.

They also discuss the molecular pathogenesis of copper in a variety of metabolic diseases, Menkes and Wilson's diseases and occipital horn syndrome, as well as the role of copper in Parkinson's disease, prion disease, familial amytrophic lateral sclerosis (ALS), and Alzheimer's disease.

Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.

Thisvolume encompasses all major methodologies to interrogate endocannabinoidsystems (ECS) and endocannabinoids (eCBs) signaling. With increasing interesttowards the manifold activities of eCBs, this book discusses the chemical,biochemical, and molecular biological assays, and activity of distinct elementsof the ECS. These include membrane, nuclear receptors, biosynthetic andhydrolytic enzymes, and membrane transporters and oxidative enzymes. Written inthe highly successful Methods in Molecular Biology series format,chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocolsis a valuable resource and will help chemists, drug designers, biochemists,molecular biologists, cell biologists, pharmacologists, and (electro)physiologists navigate the mare magnum of endocannabinoid research.

This volume provides a wide rangeof protocols used in studying the nuclear envelope, with special attention tothe experimental adjustments that may be required to successfully investigatethis complex organelle in cells from various organisms.

A comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites useful in food, environmental, and biological monitoring, and in studies of exposure via food, water, air, and the skin or lungs.

This book explores the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques. Each chapter offers step-by-step methods, lists of necessary materials, and tips on troubleshooting and avoiding known pitfalls.

Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway.

In Volume 1, Pathway Methods and Mammalian Models, assays to measure activation of the diverse Wnt signaling pathways are explored, along with a selection of models and strategies used to study mammalian Wnt/FZD fuction, from protein-protein interaction and simple cell line models to organoid cultures and mouse models.

Topics range from basic methodology and delivery to targeting and clinical targets. The authors thoroughly explain the latest developments in RNA biology, as well as the underpinnings of RNA interference, oligodeoxynucleotide delivery into cells, and strategies for targeting these molecules to accessible regions within the mRNA.

Describes techniques for studying neuronal cell death. This work features methods that solve research problems, including the detection of the key proteins involved in neuronal apoptosis, direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and detection of pro-apoptotic proteins in situ.

This detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models.

Alzheimer¿s disease (AD) and many other neurodegenerative disorders are multifactorial in nature, involving a combination of genomic, epigenomic, network dynamic and environmental factors. A proper investigation requires new integrative Systems Biology approaches, at both the experimental and computational level. The interplay of disease mechanisms and homeostatic networks will underlie the time of onset and rate of progression of the disease.This book addresses such an integrated approach to AD. It aims to present Systems Biology, including both experimental and computational approaches, as a new strategy for the study of AD and other multifactorial diseases, with the hope that the results will translate into more effective diagnosis and treatment, as well as improved public health policies.Written for the highly successful Methods in Molecular Biology series, practical and cutting-edge, Systems Biology of Alzheimer¿s Disease is intended for post-graduate students, post-doctoral researchers and experts in different fields with an interest in comprehensive Systems Biology strategies applicable to AD and other complex multifactorial diseases (including other neurodegenerative diseases and cancers). This book aims to complement other excellent volumes and monographs on AD that cover fundamental, physiological or medical aspects of the disease.

Drawing on a lifetime of teaching and practicing EKG interpretation, Dr. Blake demonstrates in An Annotated Atlas of Electrocardiography: A Guide to Confident Interpretation an orderly, confidence-inspiring method for arriving at a clinically useful interpretation.

Rising occurrences of various diseases and epidemics have pressurized the already-burdened health system across the globe, and this imposes an unprecedented challenge on our current research in identifying disease-specific biomarkers and molecular targets, in particular for cancers, neurological disorders and unexplained infertility.

Exploring the 2-D gel mapping field, the chapters in this book are separated into four different categories: Part I talks about 2-D maps reproducibility and maps modeling;

Clinical Applications of Mass Spectrometry in Drug Analysis