Bücher veröffentlicht von Humana Press Inc.

This volume describes the methods of both in vivo and in vitro electroporation using ferrets, rats, mice, chickens, and zebrafish.

In Cellular Cancer Markers leading pathologists and physicians review today's most promising cellular cancer markers, an important emerging class of prognostic markers that can be used in the clinical evaluation of cancer patients.

Plant Cell and Tissue Culture continues the high standards of Humana's Methods in Molecular Biology series. Its step-by-step approach (a hallmark of the series) is applied to a wide range of basic laboratory techniques and culture conditions appropriate to plant cells.Because of the diversity of cell types, species, and culture methods, much of this volume is devoted to the culture of particular cell types and to the regeneration of these cells into whole plants. Special attention is also given to the genetic modification of plants, as well as to the economic significance of plant products. Chapters cover a wide range of topics and techniques, including:. tissue culture media and selection . cryopreservation . callus culture techniques . organ culture . embryogenesis . batch culture . large-scale culture . hormonal control . fertilization techniques . gene transfer . cell immobilization . production systems . cell product purification . DNA expression . electrofusion of plant cells . mutant selection . mutagenesis techniques . automation . transfer of nuclei . protoplast culture . media analysis . micropropagation.A detailed appendix lists the formulas for the most commonly employed plant cell media. Comprehensive, easy to follow, and a pleasure to use, Pollard and Walker's Plant Cell and Tissue Culture is an essential tool for everyone--at all levels of proficiency and experience--involved in plant culture.

Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture.

Together with its companion volumes, In Vivo Neuromethods and In Vitro Neurochemical Techniques, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research or develop competence in complementary methods.

Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins.

" The year 1993 saw the publication of further improvements in the me- ods of naked DNA delivery and, above all, the first demonstrations by several groups of the induction of humoral and cytotoxic immune responses to viral antigens expressed from injected plasmid DNA.

Adrenergic Receptor Protocols consists of 35 chapters dealing with va- ous aspects of adrenergic receptor analyses, including the use of genetic, RNA, protein expression, transactivator, second messenger, immunocytochemical, electrophysiological, transgenic, and in situ hybridization approaches.

It goes without saying that the principles and techniques of molecular biology are having and will continue to have a major impact on investigations into nervous system structure and func tion.

Metal Carcinogenesis Testing explains fundamental principles of metal carcinogenesis as they are currently understood, and provides detailed practical descriptions of rapid and inexpensive in vitro assay methodology presently in use for the detection of potentially carci nogenic metals and their compounds.

In vitro toxicology is one of the most rapidly expanding areas of biological research today. It is hoped that the present book, In Vitro Toxicity Testing Protocols, will play some part in enabling and encouraging the more widespread use of in vitro techniques.

to the Animal Models Volumes This and several other volumes in the Neuromethods series will describe a number of animal models of neu- psychiatric disorders.

Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling.

As the technology base for the preparation of increasingly c- plex peptides has improved, the methods for their purification and ana- sis have also been improved and supplemented.

The field of neurotrophic factors has witnessed exp- sive growth in the past decade.

In a predecessor to this book, emphasis was placed on the role of protein isolation in the food industry, because at the time this reflected the interests of most of the participants at the course.

Recent advances in com- natorial library synthesis, purification, and analysis techniques are not only increasing the numbers of compounds that can be tested against each specific genomic target, but are also speeding and improving the overall processes of lead discovery and optimization.

The two Animal Models in Psychiatry volumes are loosely organized by subject. The second volume deals with affective and anxiety disorders, but also includes chapters on subjects not easily classified as either psychotic, or affective, or an- ety-related, such as aggression, mental retardation, and memory disorders.

Despite significant advances in cancer treatment and measures of neoplastic progression, drug effect (or early detection, overall cancer incidence has increased, pharmacodynamic markers), and markers that measure cancer-associated morbidity is considerable, and overall prognosis as well as predict responses to specific therapy.

In Neuroprotective Signal Transduction prominent researchers and clinicians focus on how inter- and intracellular signaling mechanisms prevent the degeneration and death of neurons occurring in both acute and chronic neurodegenerative disorders.

Thus, the function of the brain as a coordinator of vital homeostatic reflexes, and complex body reactions to external challenges, depends critically on the rate of oxygen delivery and oxygen consumption.